FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004045671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004045671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535702
Sequences flagged as poor quality0
Sequence length35-101
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN605613.9435385250523862No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAGCTCATCTCGTAT454762.9612515969895203TruSeq Adapter, Index 22 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34650.22562971201444032No Hit
GCGGCGGCGGCGGCGGCGGCGGGACCGAAACCCCCC19770.1287359136082391No Hit
CTCGGGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGC17090.11128461120712221No Hit
GCGGCGGCGGCGGCGGCGGCGGGACCGAAACCCCC16060.10457758080669297No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)