FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004045733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004045733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2730033
Sequences flagged as poor quality0
Sequence length35-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT154670.5665499281510517TruSeq Adapter, Index 6 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN57030.20889857375350407No Hit
GTCAGAGTGTAAGGAAATGAGAGGCCAGAAGGAGAGAGAAAGGAATGAGG30810.11285577866641172No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)