FastQCFastQC Report
Sat 25 May 2024
EGAF00004046053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115987905
Total Bases17.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACGATAATCTCGTA9272120.7994040413093073Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACGAAATCTCGTAT2338090.20158050100137598Illumina Paired End PCR Primer 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)