FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777147
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCT30800.39632141666891846No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG21820.2807705620686949No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG14910.19185559488745374No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG14450.1859365087943465No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCG13550.1743556881773976No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA9840.12661697207864148No Hit
GCTCAAGAACCGGCACGGCCTCGTGCCCTTCGCCTTCGTGCGCTTCGAGGACCCCCGAGATGCAGAGGATGCTAT9420.12121258912406532No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT8970.11542217881559087No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAA8820.11349204204609939No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTG8690.11181925684587343No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC7900.10165386985988493No Hit
ACCATGATCCCATCACTGGCCTCCAGGATTTCATCAAACCTCCGAACCCCCTCATGATTCTCGATTTTGCTGATA7820.1006244635828228No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)