FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872265
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG154071.766321014829209No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCT97781.1209896075160646No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC57700.6614962196121591RNA PCR Primer, Index 27 (95% over 21bp)
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT46300.530801992513743RNA PCR Primer, Index 27 (95% over 23bp)
CAAGGCCTTAACCTTAACTTTAAGAAAATCTGCGACCAGGCATGGTGGCTCATGCCTGTAATCCCAGTACTTTGG32740.3753446487019427No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG28530.32707949992261526No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA28370.3252451949808831No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG25250.289476248617106No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTG23830.27319679225923316No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTT22110.25347801413561244RNA PCR Primer, Index 27 (96% over 29bp)
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG21840.25038262454643945No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG20660.23685462560116477No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC19230.22046052518443363RNA PCR Primer, Index 27 (95% over 21bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT18220.20888147523974937No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC17270.19799028964821472RNA PCR Primer, Index 27 (95% over 21bp)
GTCCCGAAGAGTGTAAGATGTTCGCCTGCGCCAAGCTCGCCTGCACCCCCTCTCTGATCCGAGCTGGATCCAGAG16020.18365978229093222No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA15110.1732271729348306RNA PCR Primer, Index 27 (96% over 30bp)
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA14740.16898534275707497No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC14260.16348242793187848No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG13590.1558012759883751No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA13340.1529351745169186No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12210.13998039586593525No Hit
AGCTTCGTCAGACCATGGCTAAGTTCCTTCAGGAGCACCTGGCCCCCAAGGCCCAGGAGATCGATCGCAGCAATG11380.13046493898069966No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAA11320.12977707462755012No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT11130.12759883750924317RNA PCR Primer, Index 27 (95% over 23bp)
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC10970.12576453256751102RNA PCR Primer, Index 27 (95% over 24bp)
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC10870.12461809197892842No Hit
GTCTTACTCCATTGCCTAGACTGGAGTGCAGTGGTACAATCACGGCTCACTGCAGCCTCGACCTCCCAGGCTCAG10760.12335700733148756No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAA9810.11246582173995288RNA PCR Primer, Index 27 (96% over 30bp)
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG9540.10937043215077988No Hit
CCCCAGCCCCTGGGTGCTGGGAGCCCAGCTCCCCTGCCTGCTGACGCCCTGGTCTCTGCCTTCTGCTTGGAGGCT9500.10891185591534683No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG8970.10283572079585906No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)