FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961673
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG190321.9790510911713233No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCT95440.9924371381956237No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC74570.7754195033030978RNA PCR Primer, Index 39 (95% over 21bp)
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT55580.5779511330774598RNA PCR Primer, Index 39 (95% over 23bp)
AGGGAAGCGTAGCCAGCCAGGAGCAGACCTGGGGCTCCTTATTGACCTGATCTGACTCCAGGCGGCTGTGAGAGT41440.4309157062743781No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTG31470.3272422122696592No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG31180.3242266342093414No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA29340.30509331134387674No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTT24330.2529966007156279RNA PCR Primer, Index 39 (96% over 29bp)
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG24010.2496690663042427No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC21480.2233607473642288RNA PCR Primer, Index 39 (95% over 21bp)
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA20490.21306618777900596RNA PCR Primer, Index 39 (96% over 30bp)
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC19440.2021477154916484RNA PCR Primer, Index 39 (95% over 21bp)
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT18140.1886296069453962RNA PCR Primer, Index 39 (95% over 23bp)
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC14900.15493832103012148RNA PCR Primer, Index 39 (95% over 24bp)
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG14260.14828325220735114No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG13870.1442278196434755No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG12980.13497311456181052No Hit
GCATAAATCACCAAGAAGGGAGGGTTGGGCATGCAAGTAGAAATAGGGTAAGAAAGGAGTGGTTGGGAGCTGGAG10740.1116803736821144No Hit
ATGCACAACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTT10390.10804088291966188No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC10260.10668907206503667RNA PCR Primer, Index 39 (95% over 21bp)
CTGTTTGGAGTGTGAGTCACTTTAAGATCTACCAGTTGAGAGTAGTCAACCCTAGCTTCTTCAGCATGGGCCAAA9990.10388146490543043No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAA9840.10232168315009364RNA PCR Primer, Index 39 (96% over 30bp)
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG9620.10003400324226634Illumina PCR Primer Index 11 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)