FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004046458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491934
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCT37850.7694121569153586No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG29910.608008391369574No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT23130.4701850248204027No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG19270.39171921436615476No Hit
CTTCAGTGGAACATTCAGTGCACATGACTGGGTGACAGATTTCTTCCACATCTGTCTCTGCCTTCTCGGCAGCAT18500.3760667081356442No Hit
ATCACATGCTCCTTGTTCTGCAGCTTGGTGCGGATGGACATGATAACTTGGCCAATGTGAACCCTGGCCACAGTG16340.33215837896953654No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCG16250.33032886525428207No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT15510.3152861969288563No Hit
CTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCGTGATG13150.2673122817288498No Hit
CTCCAATGCCTTACCAATGTTATGAACCTGCACTTTGGCATTCTTGGGGGAGGTCCATACGTGCGGATCGTACTC12310.2502368203864746No Hit
GAGAAATCCCTGTGAAGCTTCTGGACATAGAAGAAATCCCCATGAAATTTCATGATCACCCTGGATCCATGCCTA10690.21730557351189386No Hit
GGTTTGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTCCACGTCGGTGGAATAATTCTGACGAA10610.2156793390983343No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTG10150.20632849122036698No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC10140.20612521191867203No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG9480.1927087780068058No Hit
GTGCAGTGGTCTGGTCTCGGCTCACTGCAACTTCCGCCTCCTGGGTTCAAGTGATTCTCCCTATTCAGTCTGCCA8960.18213825431866876No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8550.1738038029491761No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGCG8540.17360052364748116No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA8190.16648574808815816No Hit
GAGAAATCCCTGTGAAGCTTCTTGACATAGAAGAAATCCCCATGAAATTTCATGATCACCCTGGATCCATGCCTA8170.16607918948476827No Hit
GTCTTCAGCTGCTGCGCTGTGTTGAGCAAGGAGTTGACCATCTCTTCTAGGTACAGCCAGCTCCGCGGCTCTGAC8170.16607918948476827No Hit
GAGTACATGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAA8070.16404639646781885No Hit
ATTCTAAATACCTCATAAAGTAATTCATTTAATCTCCACAATAGTCCCAACAGGTAGGTCCTATCATAGGCTTTA7630.15510210719324136No Hit
CTGTAATCACAGCACTTTGGGAGGCAGAGGTGGGTGGATCACAAGGTCAGGAGATCGAGACCATCCTGGCTAACA7070.14371846629832458No Hit
CAGGATATTTGCCGCCTCCCCGGAGCTTATCGCAGGCTATTACGTCTTTCATCGCCTCTGACTGCCAAGGCATCC6760.13741680794578134No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG6360.12928563587798364No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC6330.1286757979728988No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA6330.1286757979728988No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAA6200.12603316705086456No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG6200.12603316705086456No Hit
GGCTTAACTGCCCCTGTCCCCCATGGAAACACGTTCTTCAGCAGAGAGAAAGGGACTCATCACAGTCATGTGTGG6130.12461021193899995No Hit
GTGCAGTGGCGCGATCTGCGCTCACTGCAAGCTCAGCCTCCTTGGTTCAGGCCATTCTCCTGCCTCAGCCTCCCG5890.11973150869832132No Hit
CTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGTGGATCACAAGGTCAGGAGATCGAGACCATCCTGGCTAACA5850.11891839149154154No Hit
GTCTAGTCATCCTCGTCGGTCTTCTGCTTCTTGGTATCGACATCGTCATCCTCATCATCTTCAGCTGCCCGCTTG5820.11830855358645673No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAA5810.11810527428476178No Hit
GTATTTGTGGAGCCACTCCACGCATGTGTCTTCCAGGTAGGCTCTCTGGTGCTCCGCCTCAGAGGCATCATTTGA5750.11688559847459212No Hit
GATATATTACTAAACTTAATGATTAAGTGGAAGTAGCACAGAAGTAGATTTGTAAAGACTCGAGAAATAGATCCT5540.11261673313899831No Hit
GGGATTACAGTGCCTGCCATCAAGCCCAGCTGATTTTTGTATTTTTAGTAGAGTTGGGGTTTAATCATGTTGGCT5420.110177381518659No Hit
TCGAGAAAGAGCTATCAATCTGTAAATCCTGTCAGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTCAAATTA5190.10550195757967533No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAC5090.1034691645627259No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)