FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004046462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380615
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG53801.4135018325604614No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCT49741.3068323634118466No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC32940.8654414565899925No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT23160.6084888929758417TruSeq Adapter, Index 11 (95% over 21bp)
GATTACAGGCATGAGCCACTGCGCCCGGCCTGTACAACTCTTTACTAGAGAAAAGTCCACTAACCTCTTGGGCTT19970.5246771672162158No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG16680.4382381146302694No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGGCAACCGATTCCGTACCCGCTGCTGGGCCTGGATAGCACCAG15620.41038845027127147No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA14700.38621704346912233No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTT14170.37229221128962336TruSeq Adapter, Index 11 (96% over 27bp)
TCCCAGGAGCCCGTGGCCACAGCCATGCCATCGTCGGTGACCCCGAGGCAGCTCACGCGGTTGTCGTGGCCAGCG13120.3447052796132575No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG12380.32526306109848535No Hit
GGCCACACATGTTTATTAGGCCGGTCCTGACACCTGCCTGCGGGGAAGGACCACCGAGACCAGATCCTGGGTGCC12290.32289846695479685No Hit
TGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCT12210.3207966054937404No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC11290.2966251986915912No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA11110.29189601040421426No Hit
CTGAAAGACAAGTCTGAATGCTCCACTTTTTCAATTCTCTCTCCATTCTTCAGTAAGTCAACTTCAATGTCGGAT11080.29110781235631805No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG9870.25931715775783926No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAA9500.2495960485004532TruSeq Adapter, Index 11 (96% over 28bp)
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAA9190.24145133533885949No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC9070.2382985431472748No Hit
GTAGCTACCGAGCTGTGCACTTGGCAGTACAACTCGTACACCAGCGGTCTGTCCATCCCGGTCCTCTCGTACTAA8920.23435755290779395No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC8740.22962836462041697No Hit
GGAATACTGGCCAGAGGGCTGTGCTGAAGTTTGCTGCTGCCACTGGAGCCACTCCAATTGCTGGCCGCTTCACTC7950.2088724826924845No Hit
ATTTCCTTTCGCCAGTCATTGAAGAAGTTCTTCCGGAAAACCTCCTTTGTTGTGTATTCATGATCTAACATCCTC7760.20388056172247546No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG7720.20282963099194726No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGA7160.1881166007645521No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT7110.18680293735139183TruSeq Adapter, Index 11 (95% over 21bp)
GTACATGGGTTCCATTCGGGTTGTTACATTCTATTCCATTCCATTCCACTCAATTCCATTGCACTCGGGTTGATT7090.1862774719861277No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAAAAAAAAAAAAAA6970.18312467979454303No Hit
ATCCAAGAGAGCGCTTCGGATTCTCAATCTATTAGTATACAACCCTACAAACCATACCTGCATACCCAGTTCTTT6920.18181101638138278No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC6790.17839549150716602TruSeq Adapter, Index 11 (95% over 22bp)
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAA6710.17629363004610957TruSeq Adapter, Index 11 (96% over 28bp)
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAAAAAAAAA6520.17130170907610054No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG6260.16447065932766708No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC6160.1618433325013465No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG6050.1589532729923939TruSeq Adapter, Index 11 (96% over 28bp)
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTAGTTCTTGATTAATGAAAACATT5690.14949489641763988No Hit
ACTGAATAGTTAGGCAAAAATATCATCACCCCTATTCAGCATGAAGTAGTTACAGAAAATGGATCTTCATCCCTC5630.14791850032184756No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT5610.14739303495658343No Hit
GCTTCGGCCTGTTGGTGAGCTTCGGCAACGCCTCGGGCCCCGTGGCGCCTTTCGCTCCTGCCGTGCTGGCGGCCA5590.14686756959131933No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG5540.14555390617815903No Hit
ATCCAAGAGATCGCTTCGGATTCTCAATCTATTAGTATACAACCCTACAAACCATACCTGCATACCCAGTTCTTT5490.14424024276499875No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGC5420.14240111398657435No Hit
GCCTGGGGTTGTGGTTATAGTCTAAACAGACTAACCGCAGTATCCTGTCTCTGTGGAACTCAGTTTGGTGGGAAT5300.1392483217949897No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAAAAAAAAAAAAAAAAAAA5290.13898558911235764No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA5280.13872285642972557No Hit
GAAGCAGATTATTCTGCCATTTTTCCAGGTCTTTGAGTTGCACGTCAAATCTGGGGATGATCACCCCACACTTGT5190.1363582622860371No Hit
GTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGAACTACAGGCATGCACCACCACGCCCGGTTAATTT5180.136095529603405No Hit
ATCCGAAGCATCAAATGAAGCAAAATTAATAAAGGCATAACCTTTGGAGTTGACTGTGTCAGGGTCCCGCATAAT5090.1337309354597165No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCA5050.13268000472918828No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAAAAAAAAAAAAAAAAAAAAAA4970.13057814326813183No Hit
CAATTTGGAGACCGAGACGTCGAAGGACGAGGTCAACGCCTACTTGCGGGACGTTTCACTGCATTCGGAACTGCA4960.13031541058549978No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC4940.12978994522023565TruSeq Adapter, Index 11 (95% over 22bp)
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAA4780.12558622229812277TruSeq Adapter, Index 11 (96% over 28bp)
GTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTTTT4640.12190796474127398No Hit
TATAGAGACACAGTGTGCGCAAGCCGAGCGCGCACCGACCCACGCCCGTCATTCACCTGTCGCAGAAACACCTGT4550.11954337059758549No Hit
TCCCAGGGGCCTGGGGTGCCTTCAGTGCCAATTGCCACTCAGATCCTTGGCTTGCCAACCACCCGAAGGCCCTGC4420.11612784572336875No Hit
TGGCAACGCCGAGGCGCTTCTGCATCTGTGGGCCGAGCATTCTTCAGGTCATCTGAACCTTCTGAGAAAACATGG4360.11455144962757642No Hit
GATGTATGGCTGTGGATGCTGACGGTACCAGAATTCTTCCTCGGCCTCCTTCAGCAGCTGGGTGGCCTTCGCCAT4300.1129750535317841No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT4300.1129750535317841No Hit
GTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT4260.11192412280125587No Hit
GAAGAATGAGTTGATGACATGCTCCATACCAGTGGCTAGATGGAGTATTAAGGTGTAGCAGAAAAGAAGTGAGAA4190.11008499402283148No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT4120.10824586524440707No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAA4020.10561853841808651No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAA3860.10141481549597363TruSeq Adapter, Index 11 (96% over 28bp)
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATACCT3820.1003638847654454No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)