FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004046467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453450
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG129110.8883002511266298No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCT90730.6242388799064296No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC56670.38989989335718467RNA PCR Primer, Index 39 (95% over 21bp)
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT45610.31380508445422955RNA PCR Primer, Index 39 (95% over 23bp)
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA30030.2066118545529602No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG24530.16877085555058652No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG24440.16815163920327497No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTG24180.16636279197770823No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTT23610.16244108844473493RNA PCR Primer, Index 39 (96% over 29bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG22810.15693694313529877No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC18960.1304482438336372RNA PCR Primer, Index 39 (95% over 21bp)
GACCAAGCTACGAAGTGTGAGAGGTCCTGAATGTAACTCATAGGGTCTCTACTGTGTTCTGTGTGCTGTGGTAGA18470.12707695483160755No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC17890.12308644948226632RNA PCR Primer, Index 39 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)