FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005715
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCT83590.8311499778764362No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG78900.7845164882695396No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCG34950.34751395773156407No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTG26220.2607100421093451No Hit
CCTTCAATGGGTGTATTTGTTTCACCATTCAGCTGGCCTTCATTCCTTCCACTGGAAAGCTCCTGTTGAATTTCC25960.2581248166727154No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGCG24060.23923278463580636No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG20980.20860780638650112No Hit
GGAATTCGGTTGCTGACCCAGCCCCAGCCTCGGCTTTCTTGTCGGCACCAGGTGGCACAGCACTCCGTCTGTAGG20970.20850837463893845No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG20830.20711633017306097No Hit
ACGAGCGACAGCGCCTGGGTGCGTTCCTCGGGATCGGTCCGCGACGCGACATAGGCCTGCACCGCGGGCGGCGCG19870.19757088240704374No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA14150.1406959228011912No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT13200.13124990678273665No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC12660.12588059241435198No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAA12530.12458797969603715No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTT12500.12428968445334912No Hit
GGAATTCGGTTGCTGACCCAGCCCCAGCCTCGGCTTTCTTGTCGGCACCAGGTGGCACAGCACTCCGTCTGTATG12320.12249991299722088No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC11200.1113635572702008No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCG10520.10460219843593861No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG10260.10201697299930894No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)