FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065695
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCT33150.31106461041855316No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG32790.3076865332013381No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG29500.27681466085512274No Hit
CTATAATACACTCATATTTGTAGAAGAAAAGCCGGGCAGTTGAATACGAATCCCAAATATTTGCTTGTTGTTACC27190.25513866537799273No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT20670.19395793355509783No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC19000.1782874086863502No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC17030.1598018194699234No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG16720.1568929196439882No Hit
ACCAAATACTGTGTGTTCTCACTTATAAGTGAGAGCTAACTTATGGGTACAAAAAGGTATACAGAGTGACATAAT14080.1321203533844111No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13760.12911761808021996No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT12810.12020324764590244TruSeq Adapter, Index 11 (95% over 21bp)
CCCTTAACCCGCAGCTGCGGATCGAGCGGCGAGCCCGGATCGTCGGCCGCTCCCATCCGCACGGTACCCGCCGGG12450.11682517042868737No Hit
GTCCCATTCAGGAAGAGGCAACCAGAACAGACCGACGGCCCCACCACAGTCGTGCGCGGGCTCCTCTGCGATGGC12300.11541763825484777No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA11880.11147654816809688No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA11220.10528340660320261No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATT11200.10509573564669066No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG10720.10059163269040391No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)