FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101840
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG167771.5226348653161983No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCT144181.3085384447832715No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC67990.6170587381107965No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT51320.46576635446162784RNA PCR Primer, Index 33 (95% over 22bp)
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA42950.3898025121614754No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG36090.3275430189501198No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTG35690.32391272780076963No Hit
CACCACCACACCTGGCTAATTTTTTTATTTTTATTTTTTCGTAGAGATAAGGTCTTACTGTGTTGACCAGGCTGG33510.3041276410368111No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTT32930.29886371887025337RNA PCR Primer, Index 33 (96% over 28bp)
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG32570.29559645683583824No Hit
CTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGGGTCAAATTAAGCCGCAGGCTCCACTCCT26270.23841937123357293No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG23020.20892325564510275No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAA22830.2071988673491614RNA PCR Primer, Index 33 (96% over 29bp)
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG22400.20329630436360996No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC21760.19748783852464968No Hit
GTCAAATGTTTAGCTTCACAGCAAAAGCTAATGAAGAGAGAATATTTAAATTTGGCCCAGAATTCTAAAATTAAA21490.1950373919988383No Hit
CACCTGAGGTTGGGAGTTCAAGACCAGCCTGATCAACATGGAGAAACCCCATCTCTACTAAAAATACAAAAAATT21400.19422057649023453No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC20960.19022725622594933No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG18380.16681187831264066No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG18030.16363537355695928No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA17460.15846220866913527RNA PCR Primer, Index 33 (96% over 29bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT16950.15383358745371378No Hit
CTAGACGTCTTTTAACCGTCTAACCGCTTGTGTCTTCTTCCGCTGATGTGCTCCTCTCGACGTTTGGCTGTCTAT15680.142307413054527No Hit
CTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCC15650.14203514121832572No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA14680.13323168518115153No Hit
ATGGAGGACCATCGGGACGAGGACTTTGTGAAGCCCAAAGGAGCCTTCAAAGCCTTCACTGGCGAGGGTCAGAAA14570.13223335511508022No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT14400.1306904813766064RNA PCR Primer, Index 33 (95% over 22bp)
GGCATCTCTCTCCCAGGTGTTTTAGTTGGGAACAAGACAGACCTGGCCGGCAGACGAGCAGTGGACTCAGCTGAG14380.1305089668191389No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCT14210.12896609308066506No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG14020.12724170478472374RNA PCR Primer, Index 15 (96% over 28bp)
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG13940.1265156465548537RNA PCR Primer, Index 33 (96% over 29bp)
AAATTAGCTGAGCACAGTGGCGCACGCCTGTAATCCCGGCTACTTGGGAGGCTGAGTCAGGAGAATCACTTGAAC13890.12606186016118492No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCTGCCGTCCCTCTTAATCATGGC12890.11698613228780948No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATAT12450.11299281202352429No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTG12050.10936252087417411No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC11710.10627677339722645RNA PCR Primer, Index 33 (95% over 23bp)
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC11690.10609525883975895No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAA11560.10491541421622014No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)