FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004046494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712926
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCT131870.7698522878396381No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG122840.7171354746206199No Hit
AGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGC57930.3381932436077215No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCG55610.3246491675647401No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTG39690.2317087836835917No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGCG37250.21746415198321467No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG36470.21291054021014333No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG25170.1469415491387252No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT22900.13368937128632527No Hit
CCTTGGAGGCCATGTGGGCCATGAGGTCCACCACCCTGTTGCTGTAGCCAAATTCGTTGTCATACCAGGAAATGA21220.123881592082787No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTT20920.12213020293929804No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC20180.11781010971869188No Hit
CTGACCCACTGTTGGACAAGGACAGCCGCCCGGCTGCCCCCTGGGCCCCCCAGGCCTGCTGGCCACAGCAGCTGG19870.1160003409370866No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC18760.10952020110617738No Hit
TGGGACCCACTGTTGGACAAGGACAGCCGCCCGGCTGCCCCCTGGGCCCCCCAGGCCTGCTGGCCACAGCAGCTG18170.10607580245731572No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)