FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004046499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288427
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCT126660.9830591876761352No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG111610.8662500863455982No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGT51010.3959091201907442No Hit
TAAACGCAGAGTACATGGGACTGAGTGCCTGCTCTGTGTCTGTATTCTGGGTGTAGGGAATATAGCAGTGAATAA45450.35275572461614046No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTG36880.2862405087754293No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCG35330.2742103355486962No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC32580.25286647982384725No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA30770.2388183420558557No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG28060.21778494241427726No Hit
TCAACGCAGAGTACATGGGACTGAGTGCCTGCTCTGTGTCTGTATTCTGGGTGTAGGGAATATAGCAGTGAATAA26720.20738466362471447No Hit
ACGCAGAGTACATGGGCCTAGAGGTTAGCTGTAACTTGTCAAAGGCCAGCCAGTGCAAGGAGGCAGAGCCAGGAT26440.20521147104182075No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG25350.1967515427726988No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG24240.18813638646194158No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT22400.17385540663149718RNA PCR Primer, Index 17 (95% over 21bp)
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG22220.17245835425677977No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCG21850.1695866354865274No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCGTG20320.15771169030142956No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG19420.15072642842784262No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT17780.13799772901375087No Hit
CGTCACCACAACCCTATGAGGTAGGAATTTCTATTATCTCTATTTCACAGATGAGGAAACTGTGGAGATCGCTAA17010.13202144941079316No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAT16750.13000348486953472No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCGTCTT14890.11556727699745505No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGG14790.11479113678927871No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA13890.10780587491569177No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTT13860.10757303285323888No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC13060.10136391118782827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)