FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153845
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCT118521.0271743605076937No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG112600.9758676425343091No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGT50660.43905377238710575No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCG35600.3085336418669752No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTG35400.3068003068003068No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC30730.26632693299359966No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA30440.2638135971469305No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG23300.2019335352668686No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG22950.19890019890019892No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG22710.19682019682019683No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCG21270.18434018434018434No Hit
GAGTACATGGGGCCAGATCAAATTTCTGCCTGTCTGCTTACCATCGCCAGCTCTCTTGGGCCCTTCACACACCTT20520.17784017784017783No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT20450.1772335105668439RNA PCR Primer, Index 17 (95% over 21bp)
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG19830.17186017186017186No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCGTG19310.16735350068683402No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG16140.1398801398801399No Hit
CTCTGGAGGTGGAGCCCAGTGACACCATCGAAAATGTGAAGGCCAAGATCCAAGATAAAGAAGGCATCCCCCCCG15530.13459346792680124No Hit
GGCCTCCACATCGGAGGTGTATGGAGGTGAGTAGGAGGCTCTCTCAGGAGATGTTCACTCCAGCCCTTGGACTGT15010.13008679675346344No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA14930.12939346272679605No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCGTCTT14570.12627345960679293No Hit
TTCCTTAGTAACGGAAGCCAAAGTTCTACCGAAGGATTTGTCTCTCATTTGTCTGGCAATTTCAATAATTTCATC14510.12575345908679242No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGG14180.12289345622678956No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTT13120.11370678037344703No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTT13000.112666779333446RNA PCR Primer, Index 17 (96% over 27bp)
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAA12990.11258011258011259No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCT12250.1061667728334395No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAT11890.10304676971343639No Hit
GCAACTCATCCTTTGCACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCA11790.10218010218010218No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)