FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852160
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG147651.7326558392790088No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCT128821.5116879459256478No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC68150.7997324446113406RNA PCR Primer, Index 27 (95% over 21bp)
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT53640.6294592564776568RNA PCR Primer, Index 27 (95% over 23bp)
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA41470.48664570033796467No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG35460.4161190386781825No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG31150.3655416823131806No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTT30950.36319470521967706RNA PCR Primer, Index 27 (96% over 29bp)
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTG28580.33538302666165976No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC22070.25898892226811865RNA PCR Primer, Index 27 (95% over 21bp)
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAA21200.24877957191137817RNA PCR Primer, Index 27 (96% over 30bp)
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC19730.23152929027412694RNA PCR Primer, Index 27 (95% over 21bp)
TGACTATTGTTGTGTATTTTTTAATACACAACAGGTGAGCCAAGGTAGCTCACTCACAGGACTGGCAAGTAGGTC17970.21087589185129554No Hit
GTACATGGGAGAAAGGCCATGTACAAGAGGAAGTACTCAGCCGCTAAATCCAAGGTTGAAAAGAAAAAGAAGGAG17330.20336556515208412No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG16700.1959725873075479No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC15770.1850591438227563No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT15640.183533608711979RNA PCR Primer, Index 27 (95% over 23bp)
ATGCTCGGCCTCGGACCGCTGCTCGGATGGGCCTGAGACTCCTTCGCCGCCGCGAGTGCCTGGTGGGCGAAGGCA15030.17637532857679308No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA14800.17367630491926397RNA PCR Primer, Index 27 (96% over 30bp)
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG13230.155252534735261RNA PCR Primer, Index 27 (100% over 28bp)
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG12950.15196676680435597RNA PCR Primer, Index 27 (96% over 30bp)
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCT12920.15161472024033046No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC12610.14797690574539993RNA PCR Primer, Index 27 (95% over 24bp)
CTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCT11680.13706346226060834No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG11350.13319095005632745No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT10770.1263847164851671No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTG9310.10925178370259107RNA PCR Primer, Index 27 (95% over 23bp)
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC9080.10655276004506196RNA PCR Primer, Index 27 (95% over 24bp)
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTG8790.10314964325948178No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTAT8600.10092001502065341No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)