FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004046517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506931
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG240341.5948971784375No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCT193041.2810141937487516No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC97220.6451522996076131No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT77450.5139585024131827RNA PCR Primer, Index 33 (95% over 22bp)
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA64220.426164170754998No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG53430.3545616886240976No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTG50060.33219835546551235No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG47880.31773186695343053No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTT47770.3170019065239218RNA PCR Primer, Index 33 (96% over 28bp)
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAA32700.21699732768122762RNA PCR Primer, Index 33 (96% over 29bp)
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC32110.213082085377499No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC28300.18779891050087894No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG26450.17552230327732324No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG26330.17472598280876828No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG26010.17260246155928838No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA24870.16503741710801623RNA PCR Primer, Index 33 (96% over 29bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG24250.1609230946871489No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG21330.1415459632856448RNA PCR Primer, Index 15 (96% over 28bp)
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT21110.14008604242662737RNA PCR Primer, Index 33 (95% over 22bp)
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG20390.13530811961529757RNA PCR Primer, Index 33 (96% over 29bp)
TATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTG20340.13497631942006635No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCT18550.12309787243078814No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA18470.12256699211841816No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTG17180.1140065470814523No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC17070.11327658665194358RNA PCR Primer, Index 33 (95% over 23bp)
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATAT16480.10936134434821501No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT15930.10571154220067143No Hit
TACCTTCTGAGAAGCACGCCAACAAAAAAATGGGGGAGTAACATAAGGAAAACAAGCAGAGCAGTTACCACACAT15420.10232718020931283No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTG15090.10013729892078668RNA PCR Primer, Index 33 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)