FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004046521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196123
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCT37021.887590950576934No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA16470.8397791182064317No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA15300.7801226781152644No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCG13640.695481916960275No Hit
GGTTTGAGGCCTGTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATG9930.5063149146199069No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTT8920.45481662018223257No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG7770.39617994829775194No Hit
GGTTTGAGGCCTGTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATC7440.3793537728874227No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT7270.37068574313058644No Hit
CGTCAGATGTGTATAAGAGACAGGCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGA6500.33142466717315155No Hit
GCAACTCATCCTTTGCACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC6100.31102930303941917No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGA5390.2748275317020441No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA5190.26462984963517794No Hit
CGTCAGATGTGTATAAGAGACAGCCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCC4920.25086297884490855No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCC4430.22587865778108634No Hit
GTATAAGAGACAGGCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA4040.20599317775069725No Hit
CGTCAGATGTGTATAAGAGACAGGGTTTGAGGCCTGTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCG3900.1988548003038909No Hit
GTGTATAAGAGACAGGCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGA3860.1968152638905177No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGC3780.1927361910637712No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTC3640.18559781361696487No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGA3470.1769297838601286No Hit
GTATAAGAGACAGCCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC3120.15908384024311276No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC3020.15398499920967965No Hit
GGTTTGAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAT3000.15296523100299303No Hit
GGTTTGAGGCCTGTTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAT2870.14633673765952998No Hit
CTGGAGAAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGC2850.14531696945284336No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAG2850.14531696945284336No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATGT2790.14225766483278351No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTT2740.13970824431606696No Hit
GCAACTCATCCTTTGCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT2670.1361390555926638No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTA2620.13358963507594723No Hit
CTGGAGAAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT2570.13104021455923068No Hit
GTGTATAAGAGACAGCCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACA2530.12900067814585744No Hit
CGTCAGATGTGTATAAGAGACAGGGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAG2460.12543148942245427No Hit
GGTTTGAGGCCTGTCACCGCCTGGGATCTCCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACG2410.12288206890573772No Hit
GCCTCAAACCCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA2180.1111547345288416No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2080.10605589349540849No Hit
GGTTTGAGGCCTGTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTC1990.10146693656531869No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)