FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153415
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG205791.7841800219348631No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCT173441.503708552429091No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC119081.0324124447835341No Hit
GAGTGAGCCTGCATGCCAATTCTAAGCTCTTCAGGATCAAATGTCGTTCGTCCTGTCCAGAATGGCAGCCTGTGG107600.9328819202108521No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT76390.662294143911775TruSeq Adapter, Index 11 (95% over 21bp)
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA60940.5283440912420941No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTT55550.4816132961683349TruSeq Adapter, Index 11 (96% over 27bp)
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG46860.4062718102330904No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG42930.372199078388958No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC40220.3487036322572534No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAA39620.34350168846425616TruSeq Adapter, Index 11 (96% over 28bp)
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC34400.2982447774651795No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAA29820.25853660651196664TruSeq Adapter, Index 11 (96% over 28bp)
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC29370.25463514866721865TruSeq Adapter, Index 11 (95% over 22bp)
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG27600.23928941447787658TruSeq Adapter, Index 11 (96% over 28bp)
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG26820.23252688754698006TruSeq Adapter, Index 11 (96% over 28bp)
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG26340.22836533251258223No Hit
CTTTAGACCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAGACCCCTTTTCTACTAAAAATGCAAAAATTAGC24080.20877134422562565No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT23570.20434969200157793TruSeq Adapter, Index 11 (95% over 21bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG22490.19498619317418275No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG22070.19134483251908463No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT21260.18432220839853825No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC19420.16836958076667982TruSeq Adapter, Index 11 (95% over 22bp)
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCT18080.15675190629565247No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT16200.14045248241092756No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAA15980.13854510302016187TruSeq Adapter, Index 11 (96% over 28bp)
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA14510.12580034072731844No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT13370.11591664752062354No Hit
GAGGAGGAATGTGGGTAGCAGTACAGTTGCGTATCTTGATGTTAGTGGTGGCCATGTAACACAAGACTACCTGAG13100.11357577281377473No Hit
GGTCCAACCGGAGCGGCCAGCATGACCAGCTGTCCAGGGGCTGCCTCCTGCCTTTTCTTTTGTAAAGACAAGACC12170.10551275993462891No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC12020.10421227398637958No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC11840.10265169084848037No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAA11780.10213149646918064TruSeq Adapter, Index 11 (96% over 28bp)
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT11610.10065761239449807TruSeq Adapter, Index 11 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)