FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753484
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG97321.2916000870622335No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCT71420.9478635246402047No Hit
TTTCAGAAGCTCATGGTCTGTTGGAGCAATAGACTTGAAGAATGTGATGAGGAGGGTATTAATTAAAAGTTGTAG32710.4341167164797129No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCG31280.4151382112957939No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGT28320.3758540327332764No Hit
GTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC22080.2930387373852663No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTG18510.24565883283520287No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG18210.24167732825116392No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC17820.2365013722919133No Hit
CTTTGGGAGTCTGAGGTAAGTGGATCACCTGAGGTCAGGAATTTGAGAGCAGTCTGGCCAACATGGTGAAACCCC15680.20809997292576882No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG14740.19562459189578016No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA14000.18580354725515072No Hit
GATCTTCAGGAAAAGCTTTTAATTTAACATATTGCTCTTCTGTCAACTCAAAGTCACGCAGGTTTCGACGAACAT12760.1693466616411231No Hit
CCGGTGGGGAGAATTATCCCCACATTGTCTCATGGAAAGACTCAACTTGCAACTGTGCCCTCCACACTATCCTTA12390.16443613932080842No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCG12020.1595256170004937No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT11660.15474781149964698RNA PCR Primer, Index 17 (95% over 21bp)
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG11310.1501027228182682No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCGTG11150.14797925370678078No Hit
TTGATATCTGGCTGTCCCCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG11120.14758110324837687No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT10720.1422724304696583No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG10190.13523843903785615No Hit
TTCTTCATCTGTCCACTGGGAAGGATCAGGATACGGAAAGTGACCCAGCACCTCTTCTGAGTCATGCCAAAAGCG9570.12700999623084233No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA9420.12501924393882285No Hit
TCCATGGAGTCTCCCCAGTGAATGCTTTCCCCGCCCAGAACAACTACGGGCTCTATGACCTCCTGGGGAACGTGT9290.12329392528573932No Hit
ATAAGGGACGACTCTCAGAAATTCTGCAGGTTAAAATATTGGTAAGGATTGGTACTGTCTCTTATACACATCTCC8930.11851611978489257No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC8830.11718895159021293No Hit
GACCAAAGAGAGCTGGCTTGGGTTGTCTTAAATCTCGTCCACAAGGCAACTCATGAAGAAGTCCTTTGCATTTCC8640.11466733202032159No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGGCG8570.11373831428404585No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAA8530.11320744700617398No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG7970.10577530511596796No Hit
CACATATTCCAAATCTTTTAGGGTAGTAAAAGCAGTGAAAATAACAAAATTATGTTCCACATGCCCAAGTCACAA7930.10524443783809609No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAT7660.10166108371246106No Hit
GGCGGAGCTTGCAGTGAGCTGAGATAGCAGCACTGCACTCCAGCCTGGGCAACACAGAGAGACTCCGTCTCAAAA7560.1003339155177814No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)