FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880210
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG230272.616080253575851No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCT132771.5083900432851252No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC76150.8651344565501415RNA PCR Primer, Index 27 (95% over 21bp)
GATCAGAGAAAGGGATCAAGGGGGTTGGGAGGGGGGAAAGAGACCAGCCTTGGTCCCTAAGCCTCCAGAAACGTC63060.7164199452403404No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT63040.7161927267356654RNA PCR Primer, Index 27 (95% over 23bp)
CTCCGGCAGCGGGAAGCTGGACCCAGGGCTCATAATGGAGCAGGTGAAAGTGCAGATCGCCGTGGCCAACGCGCA39720.4512559502845912No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA39410.44773406346212835No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG35340.40149509776076164No Hit
ACCCTGGGAAGGCTCCTGGTTGTCTACCCATGGACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCC34820.3955874166392111No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTT33800.38399927290078506RNA PCR Primer, Index 27 (96% over 29bp)
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG32750.37207030140534647No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTG32570.37002533486327127No Hit
GGTCAGGAGTTTGAGACCAGCCTGGCCAACGTGGTGAAACCCTGTCTCTGCCAAAAATACAAAAATTGGCCAGGC27350.3107213051430909No Hit
GTAGGAAGGGGAGGCCATTTTCCGTTTCTGGGAGGAGTGAGGGGCAACGGGTCGGAGAAAAAGGAAAAAAGAAGG26370.29958759841401483No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC25340.2878858454232513RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG25320.28765862691857624No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA24210.2750479999091126RNA PCR Primer, Index 27 (96% over 30bp)
TCTTCAGGCTGTGCTCTGAATGATGACAGCAATACCTTGGCCACCTCCAATGCAAGCTGATCCAACGGCATATTT21630.24573681280603493No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG20430.23210370252553367No Hit
GTTCTGTTAGTGTGGGAGGCCTCCCAGGGATATTCAGTTTTCATGTGCACATCTGCATAAACTGTCTGTGCATCA20420.23199009327319617No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG20170.2291498619647584RNA PCR Primer, Index 27 (100% over 28bp)
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC19180.21790254598334488RNA PCR Primer, Index 27 (95% over 21bp)
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT18820.21381261289919448RNA PCR Primer, Index 27 (95% over 23bp)
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC18370.20870019654400654RNA PCR Primer, Index 27 (95% over 24bp)
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG18010.20461026345985617No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA17710.20120198588973087No Hit
TTCTGATTGTCCTTGATGGACACCCGGTCCCTCTTCACCTCCTGCTCTGACCCACTGGTTTTAACAAGGATCTTG16500.1874552663568921No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAA14760.1676872564501653RNA PCR Primer, Index 27 (96% over 30bp)
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC14400.16359732336601493No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAA13480.153145272150964No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT12380.14064825439383785No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTG12270.13939855261812523RNA PCR Primer, Index 27 (95% over 23bp)
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAA12220.13883050635643768RNA PCR Primer, Index 27 (96% over 30bp)
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGA11640.13224116972086206No Hit
GGAATAGTTTTCATGCCAGGTCGTACTGATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAAT11350.1289465014030743No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCT10700.12156190000113609No Hit
GTATCAACGCAGGGTACATGGGCGGAGGTTTCAGTGAGCCGAGATCCCGCCATAGCACTCCAGCCTAGGCGACAG10610.12053941673009849No Hit
TCTTAAGGCTGTGCTCTGAATGATGACAGCAATACCTTGGCCACCTCCAATGCAAGCTGATCCAACGGCATATTT10300.11701752990763568No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC10280.11679031140296066RNA PCR Primer, Index 27 (95% over 24bp)
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC10280.11679031140296066No Hit
TGGAAAGGCTGGCCTGTTTTGGTACATGGTCTCCCTCCAGGATATGCTCTCCCACCTCCCTGGGCCCATCAGCAT10200.11588143738426057No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG9760.11088263028141011RNA PCR Primer, Index 27 (96% over 30bp)
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTG9700.11020097476738505No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC9570.10872405448699742No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC9530.10826961747764738RNA PCR Primer, Index 27 (95% over 21bp)
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT9330.10599743243089718No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAAAAAAAAA9070.1030435918701219No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG8850.10054418831869669No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)