FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764745
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG94681.2380597454053313No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCT75940.9930107421428058No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC51890.678526829204506No Hit
ATTTCTTTTTGAAAGCTTTCACAAGTTTCTTTTTGTCATAATCATCTGCAATGCCCTGAACAGTAGTCAGTGTCT37600.4916671570261983No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT34920.4566227958339054TruSeq Adapter, Index 1 (95% over 21bp)
GTGAAGCCCCATCTCTACTAAAAATATAAAAAATTAGCTGGCATGGTGGCGGGTGCCTGTAGTCCCAGCTACTCA33430.43713917711132466No Hit
GCAAAATCTATAGCTTTGTCAATTGTTTAAATTCTTCCCTAGAAGCGAAACTAATGATTTTCCATGTCTAATCAG31770.4154325951787851No Hit
GGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGA27370.3578970768033789No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA24650.3223296654440369No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG23090.30193070892912016No Hit
CCCTAGGCCCCATCCAGCCTACTCGGGTGTCAGAAGCTGGCACCACAGTTCCTGGCCAGATTCGCCCAACGATGA21610.2825778527483017No Hit
CCACCACACCCAGCTAAGTTTTTGTATTTTTAGTAGAGACGCGGTTTCACTATGTTGGCCAGGCTAGTCTCGAAC19950.2608712708157621No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTT19860.25969440793990156TruSeq Adapter, Index 1 (96% over 27bp)
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG19240.25158713035063973No Hit
GCTTAAGCAATTCTTCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCATGCCACCATACCCAACTAATTT18850.24648739122191057No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC17500.22883444808400188No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG16360.21392751832310114No Hit
CACATCCACAGCCCTTTCTAGCCTGAACACATGAATCAACAGTCTATGAGCCTTTGACCCCTAAGTTTTTAGTCT16360.21392751832310114No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG15820.2068663410679377No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC15200.19875906347867592No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA14960.19562076247638102No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA14730.1926132240158484No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAA13870.18136764542429176TruSeq Adapter, Index 1 (96% over 28bp)
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG13680.17888315713080832No Hit
CACCAGAACCGGGTGCCTGCTCTGAGCCCGTGCCCTTTAAGGCACGCACCTCTTCTCAGGGGTTCCCCATTCTGA13040.17051435445802196No Hit
CAAGGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCG12110.15835343807412927No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAA11660.15246912369482638No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAA11380.14880777252548233TruSeq Adapter, Index 1 (96% over 28bp)
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC10880.142269645437368TruSeq Adapter, Index 1 (95% over 22bp)
ATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAG10720.1401774447691714No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG10470.13690838122511426No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT10340.13520846818220453No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT10320.13494694309867994TruSeq Adapter, Index 1 (95% over 21bp)
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGA10290.1345546554733931No Hit
GACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG10060.13154711701286048No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA9740.12736271567646731No Hit
GCCCGTTAGTGGCCACAGTCACATGATTGATTACCGGTTGGGGACCCACAGTTAGCTTATTGCAGAACTGCATCT9540.12474746484122158No Hit
TGGGACCCACTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCG9260.12108611367187755No Hit
GGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAG9140.11951696317073011No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC8620.1127173109990912No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG8470.1107558728726569TruSeq Adapter, Index 1 (96% over 28bp)
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC8410.10997129762208317No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC8320.1087944347462226TruSeq Adapter, Index 1 (95% over 22bp)
CAAGGCCCCATCCAGCCTACTCGGGTGTCAGAAGCTGGCACCACAGTTCCTGGCCAGATTCGCCCAACGATGACC8320.1087944347462226No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG8310.10866367220446031No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATGTCGTATGCCG7910.10343317053396885No Hit
GCCCTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTT7690.10055639461519852No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)