FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054111
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG167171.5858861163577649No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCT87870.8335934261192607No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC63240.5999368187980203RNA PCR Primer, Index 39 (95% over 21bp)
CATATTTGGAATGACTATTAAAAAAACAACAATGTGCAATCAAAGTCCTCGGCCACATTGTGAACTTTGGGGGAT49900.4733846814993867No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT45390.4305998134921275RNA PCR Primer, Index 39 (95% over 23bp)
ACAGGTGCTACGCATGATGGTCGGAGTAAATATCTCAGATGAGCAGCTGGGCAGCATCGCAGACAGGACCATTCA33640.31913147666611963No Hit
CCATGGTGAAACCCCATCTCTACTAAAAATACAAAAAAATTAGCCGAGCATGGTGGCGTGCACTTGTAGTCCCAG32940.31249080979137867No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTG26620.25253507457943236No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG26270.2492147411420619No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA25830.24504060767793906No Hit
ATCCGGGAGTGGGTAATTTGCGTGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA25690.24371247430299087No Hit
GAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCACAGAGGAGGACAAGGCTACTATCACAAGCC23140.21952147354500615No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAA22300.21155267329531707No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCGCTCAGCGGCCATCACAATGCTTTGTTTT21650.205386339768772No Hit
GACGTCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCG20890.19817647287619614No Hit
GTGTCAACACCGCTCAGCGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCGGGGTCCGTGCCAGT20870.19798673953691787No Hit
GGCACTGTAAAAGTTTTAATTCCTTCCAGAAGAACCAGTGGAAGAATTTAAATTTGGCGCTACGATCAAAACTAC20840.1977021395280004No Hit
CTACCTCCCCTATCCGGGAGTGGGTAATTTGCGTGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT19520.18517973913563182No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG18620.17664173886810783No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC18070.17142407203795426RNA PCR Primer, Index 39 (95% over 21bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG17840.16924213863625368No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT16890.16022980502053388No Hit
CCCCTATCCGGGAGTGGGTAATTTGCGTGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT16520.15671973824388513No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCA16170.15339940480651468No Hit
CAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACT14940.14173080444089853No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT14940.14173080444089853RNA PCR Primer, Index 39 (95% over 23bp)
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC14750.1399283377177546RNA PCR Primer, Index 39 (95% over 21bp)
TTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGC14720.13964373770883712No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG14510.13765153764641486No Hit
TGCTGGCGCTGAGTACGTCGTGGAGTCCACTGGCGTCTTCACCACCATGGAGAAGGCTGGGGCTCATTTGCAGGG13540.12844947069141677No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC12730.12076527045064514RNA PCR Primer, Index 39 (95% over 24bp)
AACTACAGCCTCCAACTCCTGGGCTCAAGAGATCCTTCCACCTCAGCCTCCAGAGTAGCTGTAGATGTGCATGAC12600.11953200374533611No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTT12360.11725520367399639RNA PCR Primer, Index 39 (96% over 29bp)
GGACATACCCTTCAAGTGTCTGCCTTATCAACTTTCGATGGTACTCTATATGACTACCATGGTGATAACGGGTAA12200.1157373369597699No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG11490.10900180341538984No Hit
CCACAAGTCCCAGAGAATAGTGGGAAAGAAAGTTGGGTGGTTGCAGCTCAGCCCCAAGAGTTCAGGGAGGAAAAA11030.10463793661198868No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA10880.10321493656740134RNA PCR Primer, Index 39 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)