FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004046539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104332
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCT22442.150826208641644No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA11111.0648698385921866No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG8150.7811601426216309No Hit
CGTCAGATGTGTATAAGAGACAGGCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGA5190.4974504466510754No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG4830.4629452133573592No Hit
CGTCAGATGTGTATAAGAGACAGCCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCC4000.3833914810412912No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAT4000.3833914810412912No Hit
GGTTTGAGGCCTGTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATG3440.3297166736955105No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGA3260.3124640570486524No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA3240.3105470996434459No Hit
GTGTATAAGAGACAGGCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGA2980.285626653375762No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCC2940.2817927385653491No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGC2880.2760418663497297No Hit
CGTCAGATGTGTATAAGAGACAGGGTTTGAGGCCTGTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCG2820.27029099413411034No Hit
GTATAAGAGACAGGCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA2810.2693325154315071No Hit
CTGGAGAAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGC2750.26358164321588773No Hit
GGTTTGAGGCCTGTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATC2740.26262316451328455No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTC2570.24632902656902966No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAG2360.22620097381436186No Hit
GCAACTCATCCTTTGCACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAG2250.21565770808572635No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATAT2170.20798987846490052No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTT2050.19648813403366178No Hit
GGTTTGAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAA2040.19552965533105854No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATGT2010.19265421922324888No Hit
GGTTTGAGGCCTGTTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAA1840.176360081278994No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGA1840.176360081278994No Hit
CTGGAGAAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT1750.16773377295556494No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC1630.1562320285243262No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG1630.1562320285243262No Hit
GCCTCAAACCCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA1610.15431507111911974No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1590.15239811371391326No Hit
GTATAAGAGACAGCCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG1580.15143963501131005No Hit
CGTCAGATGTGTATAAGAGACAGGGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAG1530.1466472414982939No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCC1460.1399378905800713No Hit
GGTTTGAGGCCTGTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTC1460.1399378905800713No Hit
GTGTATAAGAGACAGCCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACA1410.13514549706705517No Hit
GATGTGTATAAGAGACAGGCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCA1410.13514549706705517No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCG1400.13418701836445196No Hit
GTACATGGGTGGCGGTCGCATTGGCGGCCACCGCATTATAGACGTTCAGCGCGCGGGTGGTGCTGCGCCCCGCCA1270.12172679523060999No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1270.12172679523060999No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT1260.12076831652800674RNA PCR Primer, Index 33 (95% over 22bp)
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT1240.1188513591228003No Hit
GTATCAACGCAGAGTACATGGGTGGCGGTCGCATTGGCGGCCACCGCATTATAGACGTTCAGCGCGCGGGTGGTG1220.11693440171759382No Hit
CGTCAGATGTGTATAAGAGACAGCAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACAT1180.11310048690718093No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACAC1180.11310048690718093No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCG1140.10926657209676802No Hit
GCAACTCATCCTTTGCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAT1130.10830809339416479No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTA1070.10255722117854542No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCT1070.10255722117854542No Hit
GTATAGACCAGCGCCGATTCACACACCGCGCCGGTGGAGGACAGCACCTCGGCGAACAACAGGAAGGCGAGCGCG1060.10159874247594218No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)