FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992179
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACAAAACAGCTGCCCAGGCAAGGCGTAGGTTAATGAGTGGTAAAGTCAAGGTCTGGGGGAATGTTGGAACTGT73220.7379716764817639No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG50630.5102909857999414No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCT39970.4028506952878462No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC34970.35245656277748266No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT25480.2568084992728127Illumina PCR Primer Index 10 (95% over 21bp)
GGAAACCCTGCAGGCAGTCACAGCTCTAAGCCTCCTTTCTCACAACATCCAGCACCGAGTCCACCAGCTCCGCGC23110.23292168046290035No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG20670.2083293437978429No Hit
GCCTACTCCCCGACTTCTTGCTGGAGCGCCGCCTCACGCTAGCCGATGCCCTGGAAAAGTGCCTCAAGAAAGGGA17410.1754723694010859No Hit
CTGTCAGCCAGGTTGGAGTGTAGTGGACGATGTTGGCTCACTGAAACCCCCACCTCCCAGGTTCAAGTGATTCTC16100.16226910668337063No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG16040.16166437709324627No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT15490.1561210225171063Illumina PCR Primer Index 10 (96% over 27bp)
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG12290.12386877771047361No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTG12130.12225616547014198No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT11580.11671281089400198No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC11550.11641044609893982No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA10690.10774265530715728No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA10410.10492058388657692Illumina PCR Primer Index 10 (96% over 28bp)
ATAGAAAGGAGAGACAATTATGTTCCTGAGGTCTCAGCCTTGGATCAGGAGATTATTGAAGTAGATCCTGACACT10180.1026024537911002No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)