FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004046614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2488000
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCT49040.19710610932475886No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG42550.1710209003215434No Hit
GAGCCAGGGGGAAATTCCACCACTGGGGTGCAATGAAGGAGACGGAGGCAGAGCAGCCTGAGAAGCAAGAAGAAT38940.1565112540192926No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC27440.11028938906752413RNA PCR Primer, Index 29 (95% over 21bp)
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTG26720.10739549839228295No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT25200.10128617363344053RNA PCR Primer, Index 29 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)