FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004046616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288087
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCT78180.6069465804716607No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC69410.5388611173003066TruSeq Adapter, Index 11 (95% over 21bp)
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG58200.4518328342728402No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT47520.36891918014854586TruSeq Adapter, Index 11 (95% over 23bp)
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT35150.2728852942386656TruSeq Adapter, Index 11 (96% over 29bp)
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG24990.19400863450993605No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA22700.1762303322679291No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG22550.17506581465382387No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG20390.1582967610107081No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG19280.1496793306663292No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC18990.1474279299457257TruSeq Adapter, Index 11 (95% over 21bp)
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC18670.14494362570230118TruSeq Adapter, Index 11 (95% over 21bp)
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA18600.14440018414905204No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAA16480.12794166853636438TruSeq Adapter, Index 11 (96% over 30bp)
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT15380.11940187269959249TruSeq Adapter, Index 11 (95% over 23bp)
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG14680.11396745716710129No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC14530.11280293955299603TruSeq Adapter, Index 11 (95% over 24bp)
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA14400.11179369095410481TruSeq Adapter, Index 11 (96% over 30bp)
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTG14020.10884357966503816No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)