FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978551
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCAGCGCCAAGGACATCAAGAAGATCTTGGACAGCGTGGGTATCGAGGCGGACGACGACCGGCTCAACAAGG127221.3000855346323288No Hit
GTTCACAGATGAAGAGGGATATGGTCGTTATCTCGATCTCCATGACTGTTACCTCAAGTACATTAACCTGAAGGC119981.2260985886274707No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCT55880.57104841750711No Hit
GAATTGGCAGTCCCAGCACAGATGCCAGTGCCAGTGATGTCCACGGCAATTTCGCCTTCGAGGGCATTGGAGATG52790.5394711159663625No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG49880.5097332688842994No Hit
CAGGTGTGTACCACCGTGCCCGGATTTTTTTTATTCTTTATTTTTTGTAGAGATGGAGGGATCTCACTTTGTTGC47990.4904189970681139No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC42340.43268056544830064No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT32680.3339631761655754TruSeq Adapter, Index 12 (95% over 22bp)
CTCTGTCACCCAGGCTGGAGTACAGTGGCGTGATCTTGGCTCACTGCAATCTCCACCTCGTGGGTTCAAGCGATT27290.27888173431941715No Hit
TCCCAAGCCTGTGAGCAGGATGCGCATGGCCACCCCGCTGCTGATGCAGGCGCTGCCCATGGGAGCCCTGCCCCA27170.27765543134696097No Hit
CCTCCAGCGCCAAGGACATCAAGAAGATCTTGGACAGCGTGGGTATCGAGGCGGACGACGACCGGCTCAACAAGG26790.27377213860084965No Hit
GATGTCACCTGGGTGCAGGGCCCGTCCCTCCGTGAGTCACAGCCCGGCAGAGCATACCAGGCAGGACACCTCCAC26700.27285241137150745No Hit
CTGTCATTGTGCATCTCTGAGGTTCCCTCATGGAGCTCCACAGATCCATTTTTAGGGAAGGGATTTTGGCTCAAA25510.26069157356131667No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG23250.23759620091339134No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG22120.22604851458942868No Hit
GGCACCAAGGCTGGAGTGCAGTGGCACCATCTCAGCTCACTGCGGCCTTGATCGCTTCAGGCGCAGGTGATCCTC21710.22185864610020328No Hit
CTTCTCCAGCGCCAAGGACATCAAGAAGATCTTGGACAGCGTGGGTATCGAGGCGGACGACGACCGGCTCAACAA19220.19641285942173684No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTT18830.19242737476125413TruSeq Adapter, Index 12 (96% over 28bp)
CTTTTTAATCAATGAATTTGTTTCACCAGTGGATAGTTCTGAAAAATGGAAAGTGTCCAAATCAGTATCTCGCAG18540.18946380924448497No Hit
CTGACCAGCACCATGGCGGTTGGCAAGAACAAGCGCCTTACGAAAGGCGGCAAAAAGGGAGCCAAGAAGAAAGTG17940.1833322943822039No Hit
CTGCACACCTGAGCATGTGTGCATGTGTGTGCCTTTTTGTGTGAGTGAATGTGTGCCAGGGGTACTTAGATGGGG17760.18149283992351956No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT17320.17699639569118011No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA17160.1753613250612385No Hit
AACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCAT15690.1603391136486499No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG15670.1601347298199072No Hit
AGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGC15610.15952157833367908No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA14630.14950677072528668No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG14450.14766731626660237No Hit
GCTTCATTCCGCATCAGGTGCAGCTCCCGGAATTTGCGCAGTCTCTGTTCGCGCTTCTGAGCGGCCAGCTCCGCC13870.14174018523306398No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTG13840.14143360948994993No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCATTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC12880.1316231857103002No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGC12690.12968153933724455No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC12350.1262070142486186No Hit
CTCCTGCCCAGCTGAGCTGCCCAACATCAAACGGGAGATCTCTGAGACCGAGGCAAAGGCCCTTTTGAAGGAACG11990.12252810533124997No Hit
CAATGAGGCTGGGCGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGAAGATCACCTGAG11950.12211933767376458No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC11340.11588563089711217No Hit
CACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG11230.1147615198390273No Hit
CGATAAAGCCGTTGGTGTGAGATAAATATCGAGAGGTCTTTCCCTCATACCACAGAGTAGAATCTTGGGTCATCT11140.11384179260968515No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGA10770.11006069177794514No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAA10510.10740370200429002No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC10320.10546205563123434No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAA9960.10178314671386571No Hit
CCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCCCAGCTCCACCCTCA9920.10137437905638032No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)