FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770143
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCT18780.24385081731574526No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG17720.23008713966107594No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG17360.22541268309911275No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC14970.19437948536830174No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT12130.15750321693503674TruSeq Adapter, Index 12 (95% over 22bp)
CGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTC11880.15425706654478452No Hit
CAGAAACTGTCTCTTGCCAATACCTTTAGTACTGGAATTCTCTCATCTTCCTCAACAGTCTTGGCAGCTAGGAAA10540.13685770045303275No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT10350.13439062615644107No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC9530.12374325287641387No Hit
ATATGGGGACCAACATGTGATGGCCTAGATCGGATTGTTGAGCGCTGTGACCTGCCTGAAATGCATGTGGGTGAT8540.11088849733101515No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG7980.10361712045685022No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)