FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011461
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG50490.49917891050668295No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCT42840.4235457422480946No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC38250.3781658412929416No Hit
GGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACG29610.29274485125971245No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT29360.2902731790943991Illumina PCR Primer Index 10 (95% over 22bp)
CAACAGCAGTTGGACGTGGGTTAGTCGATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTGATTTTATGCAG25110.24825475228407223No Hit
CAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAA24310.24034540135506954No Hit
GGTCAGTGCTCAGTGATGCCAAATTAAGTAAAAGGCACAGGGACTAGATGTTTCAAAGCAAGGTTAGAGGGACCA24000.23728052787008103No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG23560.23293038485912954No Hit
AACTAGGGCTGGCCAATTCCTAAAGATAGTAAAAACCTTGCCAGCTCAACTAGCAGGCTTTTAAAATGCAAACCA22570.22314256308448868No Hit
TCCCAGCACTTTGGGAGGCCGAGGCGGGTGGATCATGAGGTCAGGAGATCGAGACCATCCTGGCTAATATGGTGA18850.1863640812646261No Hit
AACAAGTTCTATCATCAAGAAGAAGAGCTGGAGTCAGCAGATTGTTCTCATCTCTTCCCAACTCAGCATTCAGTG18820.18606748060478853No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTT17360.1716329151593586Illumina PCR Primer Index 10 (96% over 28bp)
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG16950.1675793728082447No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15730.1555176126415156No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTG15530.15354027490926492No Hit
TTCTGGAACACTTCTGCAACATCTTTCACATTCATGAGCTCGAAGTAAAGCTTCAGCAGTAAAGAGAGGAGCCTG14420.14256605049527368No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT13900.1374249723914219No Hit
ATGTCTCTGAGCCCCAAGCCCCAATCTTCCCATCTGTCAAATGGGGATGATGTGTGTGGCGCGGATGTCCGGGGT13310.13159182608128242No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG12290.12150740364680397No Hit
CGCCCAGCGAGTTCCAGAGGCGCCAGTGGAAGCTGCGGCGGCGGTGTCTCGCGTTCGGCGGGATTTCTCTTCGCT12160.12022213412084104No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA11760.11626745865633968Illumina PCR Primer Index 10 (96% over 29bp)
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG11740.11606972488311462No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC11580.11448785469731408No Hit
GTGACATGGTGATGGCCACAGTCAAGAAAGGCAAACCAGAGCTCAGAAAAAAGGTACATCCAGCAGTGGTCATTC11560.11429012092408902No Hit
TCCTAGACTCGAAGGTATGAGGTCAAATAAAGCTGATTTTTTCAACTGCCCATATTTACATCACCTTATAAATAG10970.10845697461394953No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA10860.10736943886121164No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA10470.10351363028332285No Hit
CAACAGCAGTTGGACGTGGGTTAGTCGATCCTAAGAGATGTGGAAGCTCCGTTTCAAAGGCCTGATTTTATGCAG10290.10173402632429723No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)