FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004046636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429372
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCT86080.6022225145028726No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG84540.5914485522313295No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC66200.4631404560884081No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT47440.33189400659870205Illumina PCR Primer Index 10 (95% over 21bp)
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT39300.27494592030626036Illumina PCR Primer Index 10 (96% over 27bp)
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG26090.18252771147049193No Hit
AATCAATTTCATGGCAGCAACTTGTTCCAGTTAAGTAGAGCACATCAGGAAAAAATGGACGGTCACAGATTATAG26010.18196802511872345No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA24690.17273320031454373No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG23960.16762606235465646No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTG22880.16007029660578212No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC18290.1279582921730662No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG17840.12481005644436859No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC17590.12306103659509211No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAA17360.12145193833375777Illumina PCR Primer Index 10 (96% over 28bp)
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT16010.11200723114766485Illumina PCR Primer Index 10 (95% over 21bp)
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA14830.10375185745907993Illumina PCR Primer Index 10 (96% over 28bp)
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG14440.10102338649420864No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)