FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914990
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG29300.32022207892982435No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCT21530.23530311806686413No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG21180.23147793964961366No Hit
GATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGTCGTTCTTAGTTGGTGGAGCGATTT19400.21202417512759703No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG17400.1901660127433087No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA16150.17650466125312844No Hit
TCATTCCCTCTTTCTCTCTTGTAATGGTTGGACCCTATTCTGAAATATATATTTTAAGATAGTGTTACTTTGGGT15970.1745374266385425No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT15940.17420955420277817No Hit
CAGCCTAACTTTGGGAAATTTTAAAACATATACTGAGAGCATGGGGGAGTAAAATGAACCCCCATGTACTCATCA15700.17158657471666355No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC15490.16929146766631328No Hit
CTTCAAACTTCCACAGATTCCTTGGGAAAGGGCACAATGCAACCAAGCTGTTTGCTAAGGCATAACATGTTATGA13820.15103990207543252No Hit
TAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCCCCTTGCCTCTCGG12370.13519273434682347No Hit
CCTCCGCTCCACCGACCGCGGGCCGCGCTCACTCGCTGGGCCTTCTCCCACAGCTGGTTCAACTTCTCCATGCGG12190.1332254997322375No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTG12060.13180471917725878No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10740.11737833200362846No Hit
GTGCCTGGAGGAGGTGCAGTCCCTGCCCCTACCCCTATGAGGGGCTCCGGTAGCACCTGGGCACCTGCCGCTGGA10650.11639471469633547No Hit
GACAGGCATTTAGCTGGGAGACAATTTTGGTTTAGGGCTCCTAGGCTGTTCAGTGAATAGGCAAGAGGTAACATA10450.11420889845790663No Hit
CTAACAGCTGTCCAGTGTTGGTGATTCCAGTGGACTGTATCTCTGTGAAAAACACAATTTTGCCTTTTTGTAATT10380.11344386277445655No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG10140.11082088328834194No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA10010.10940010273336322No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGT9430.10306123564191957No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)