FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004046641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622980
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGC14240.22857876657356577No Hit
AACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGG13400.2150951876464734No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG10740.1723971877106809No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGTCTGCCGTTACTGC10490.16838421779190343No Hit
TCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGT10070.16164242832835726No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT9550.15329545089730007No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG9310.14944299977527367No Hit
CTCCAGAGCCAGTGCACATTGATGCATTTCAGTTGCCCTTGAGGACTTCCAAATCTGACATTGGAGGAGAGAGTG8590.1378856464091945No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCT8450.1356383832546791No Hit
GTCTCTGGCCAGCACGCTGGACTGCGAGACGGCCCGGCTGCAGCGAGCGCTGGACGGAGAGGAAAGCGACTTTGA7920.12713088702687086No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG7730.12408102988859995No Hit
AAATAATGTCAAGCAAATACAAGTTCAAGATGTGGCCATAGGAATAGTTGCTATACATTGGTAGAGTTCTAGGTC7590.12183376673408457No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC7410.11894442839256478No Hit
GTATCAACCTGAAGAGGGGGTGAATCCCAGCAGCTGCTCGATGGGCTTAAACCGCCACTCGTCAGCCTCCAGCTC7340.11782079681530706No Hit
CAGGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAG6810.1093133005874988No Hit
GGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACC6580.10562136826222351No Hit
AAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCTGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT6560.1053003306687213No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)