FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842090
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC87061.0338562386443255No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG83600.9927679939198898No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCT66340.7878017789072427No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT65070.7727202555546319RNA PCR Primer, Index 47 (95% over 22bp)
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG42330.5026778610362312No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT37050.43997672457813297RNA PCR Primer, Index 47 (96% over 28bp)
CGCGCGAATCGGATGCCAATTTCATCGCGATCAAATCGTCGGATCTGCTGTCGAAATGGTATGGCGAAAGCGAGC33050.3924758636250282No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA29170.3464000285005166No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAA25880.3073305703665879RNA PCR Primer, Index 47 (96% over 29bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG25450.3022242278141291No Hit
GGTAATTCTAGAGCTAATACATGCCTACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCA22750.2701611466707834No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG22690.2694486337564868No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT22350.2654110605754729No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG22180.26339227398496595No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC22070.26208600030875556No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC21710.2578109228229762No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAA20850.24759823771805864RNA PCR Primer, Index 47 (96% over 29bp)
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC19140.22729161966060632RNA PCR Primer, Index 47 (95% over 23bp)
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAA18700.22206652495576482No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGA17470.2074600102126851No Hit
AACTTGAGTGGTTAACAAAATAGCAAATCCCTTACTGAGTGTTCAATGTTGTAGTAAGAAATTCCTCACTAGAAT17060.20259117196499188No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT16980.20164115474592975RNA PCR Primer, Index 47 (95% over 22bp)
AACCACCACACTTGGCTAATTTTTATATTTTTAGTAGAGATGAGGTTTCACCCTGTTATGTTGGCCAGCCTGGTC16710.1984348466315952No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA16550.19653481219347102No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC15670.18608462278378796No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG15210.1806220237741809No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG15010.17824698072652567No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAA14900.17694070705031528RNA PCR Primer, Index 47 (96% over 29bp)
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC14720.17480316830742557No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT13960.16577800472633566No Hit
CTATGGGTGCAGTCATTTGATGATAGCCACCTTTTGGGACTCCATTGTGGGCAAGGCAGGCATGGTAAATCCATC13930.16542174826918737No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCA12880.15295277226899737No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAAAAAAAAA12100.14369010438314195No Hit
CAGCCCATATCCTGAATGGACATCAGGAAGCCCAATAGATCGATGAACAATTCCAGGCAGGGCTGCCACATTGCC12050.14309634362122814No Hit
CACGGATGGCGGTGGCCACCTCCTTGGAGCACTTAACACCCAGACCGACGTGGCCATTGTAGTCCCCGATAGCAA11810.14024629196404184No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAAAAAAAAAAAAAA11620.13799000106876935No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGA11470.13620871878302795No Hit
CAGAAATAGTGCCACATATCTACAACCATCTGATCTTTGACAAACCTGACAAAAACAAACAATGGGGAAAGGATT10940.12991485470674158No Hit
GAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGT10820.12848982887814842No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTG10790.12813357242100015No Hit
CCCCATCCCCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGCTGTCTC10460.124214751392369No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAA10440.12397724708760346RNA PCR Primer, Index 47 (96% over 29bp)
GTCCACCTCAGAGGGCAGAGTATCATCCACCAGAATGTTGGGGCCAGTCGCATCAGGGCCAAAAGCCCAGATGGA9680.11495208350651355No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA9590.11388331413506869No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG9530.11317080122077214No Hit
GTCAGAGGGGAACAGCCAGGTCTGCCAGAACGGTAGGCCATCCCCGGGCCTGCATCCCCCATGGCGGGCTCTTTC9230.10960823664928926No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAA9170.1088957237349927No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT9060.10758945005878232No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAAAAAAAAAAAAAAAAAAA9020.10711444144925125No Hit
CAACACCTGTGGTTTCAAAGAAAAGTTCCCACAGAGCGGATACACTACTCGTCCAGATTAATCGGCCATCCCACC8480.10070182522058213No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)