FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004046646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235116
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG33360.27009608814070907No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG27260.22070801446989596No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCT23360.1891320329426548No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGT22620.18314069285799878No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTG22300.18054984309166103No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG21620.17504428733819336No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCG20730.1678384864255665No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT19690.15941822468496886No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC18530.15002639428199457No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTT16150.13075694914485764No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTG15890.12865188370970823No Hit
GAAAAAATGATTTCACAGGGTAACAATAACAAAAATGGAAAGAATGAGACTGGTAATAACAACAACAAAGATGGA15390.12460368094980552No Hit
CTCATAACCAGTTCATCAAGATGATGATCATCTTTTCCATCGCCTTCATCACTGTCCTTATCTTCAAGGTCTACA14230.11521185054683124No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA14210.11504992243643512No Hit
CCTCCACCTACATCGGCTCCTTCTCCCAGGCACCAGGGCCAGGGACACCCCTGTTGAGCTCCTGGCACCACTGCC13810.11181136022851296No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC13750.11132557589732463No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT12900.10444363120549001TruSeq Adapter, Index 5 (95% over 21bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12670.10258145793593478No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)