FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721383
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG71730.9943400385093633No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCT63880.8855212834236459No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC62460.8658368716756564No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT43350.6009290487854579TruSeq Adapter, Index 12 (95% over 22bp)
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTT28740.39840140396987456TruSeq Adapter, Index 12 (96% over 28bp)
AATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAA26780.3712313708529311No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG21410.2967910250172239No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTG19140.2653236907440292No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG18750.25991740864422924No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG18440.255620107487978No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA16320.2262321124839371TruSeq Adapter, Index 12 (96% over 29bp)
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC16150.2238755279788961No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG16110.22332103750712173No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA15270.21167673759986028No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAA15130.20973602094865001TruSeq Adapter, Index 12 (96% over 29bp)
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC14910.2066863233538911No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT13580.18824951516739374TruSeq Adapter, Index 12 (95% over 22bp)
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC11490.15927738801718366TruSeq Adapter, Index 12 (95% over 23bp)
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAA10260.14222680601012222TruSeq Adapter, Index 12 (96% over 29bp)
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG10250.14208818339217863No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGT10190.14125644768451712No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC10160.14084057983068632No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT10100.1400088441230248No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA9990.13848399532564531No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGTC9350.12961214777725563No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGTCTT9270.1285031668337069No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCG8940.1239286204415685No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG8590.11907682881354287No Hit
GACAGGCTCAGGGATGACCTTGCCCACAGCCTTGGCAGCGCCAGTAGAGGCAGGGATGATGTTCTGGAGAGCCCC8440.11699748954438903No Hit
CCCCTACACAGTTCTAGTAAAACTAAATGAAACAGTGTGAAGCTCCTAGCTTTATCTGAACTCCCATGTCTGAAA7860.10895737770366089No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGTCTTCTGCTT7490.10382834083974808Illumina PCR Primer Index 12 (96% over 26bp)
AATTGGGTGTGAGGAGTTCAGTTATATGTTTTGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAA7420.10285798251414298No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)