FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004046680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2288677
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG103880.45388667776186853No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC96870.4232576287523316No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG75680.3306713878804218No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCT73060.31922372619640077No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT71510.31245125458944184RNA PCR Primer, Index 47 (95% over 22bp)
GCCTAGACATGAGGAATAGGGAGAAAAGAGTTCCATCTGAATTTTAAAGACCTCTTTCTGTACTCCAAGGGATTG66550.2907793454471732No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG49470.2161510776750061No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC44500.1944354751675313No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT42960.18770669692577852No Hit
GTGCAGTGCTGCAATCTGGGCTTACTGCAGTCTCTATCTCCTGGGCTCAAGCCGTCCTTTGACCTCAGCCTCCTG39130.17097213805180897No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT38180.16682126835722122RNA PCR Primer, Index 47 (96% over 28bp)
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA37480.16376273279278813No Hit
TCCACCCCCTAGCAGAAAATAGCCCACTAATCCAAACTCTAACACTATGCTTAGGCGCTATAACCACTCTGTTCG36900.16122851761082932No Hit
GTATGAAGCCAAATTGGGAGATTAAGAAAAGAACAAAGCCATAAATCGCTCTTTCTGGCAATGGCGACGGTGAAT32780.14322685114588035No Hit
TCCACCCCCTAGCAGAAAATAGCCCACTAATCCAAACTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCG28800.12583689179381802No Hit
CTCATTTCTGAACACCTGGATTGTAGCTTTCCCTGGACTGACTGTCAAAGGAGGTGGTTTTAAAAAGCAAGCCAA28420.12417654391598291No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA28210.12325898324665298No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAA28050.122559889403354RNA PCR Primer, Index 47 (96% over 29bp)
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG27880.1218171021948488No Hit
CTTCTTCATTCTTCAGGAATTATTTATTCACACAGCAGACCACCATTTCATATTTTAAATCCTAGGGCTCTCATG27020.11805947278711675No Hit
GTGCAGATCCCGGAATGCCTGAACCAACTGTTTTGAGCCTACTTTGGGGATAAAGGATTATTTGGTCTTCTGGAT26890.11749145903943632No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA26720.11674867183093114No Hit
ACGCAGAGTACATGGGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTT25970.11347166944046713No Hit
GTATGAAGCCAAATTGGGAGCTTAAGAAAAGAACAAAGCCATAAATCGCTCTTTCTGGCAATGGCGACGGTGAAT25850.11294734905799289No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG25270.11041313387603405No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC24940.1089712528242299No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG24110.10534470351211639No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAA23670.10342219544304417RNA PCR Primer, Index 47 (96% over 29bp)
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC23660.10337850207783798No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAA23210.10141230064355958No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)