FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004046689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245689
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAG98840.7934564726829891No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG97350.7814952207172096No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCT87420.7017802998982892No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC87320.7009775313099819No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGC67630.5429123962722637No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT65330.5244487187411946TruSeq Adapter, Index 12 (95% over 22bp)
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTT44060.35369984000822036TruSeq Adapter, Index 12 (96% over 28bp)
CTACTTTCCTCAGGTGATCCATACGTCTCTGTCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGTGCCCGG36240.2909233364025852No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT33510.2690077539417945No Hit
GTTCAAGTACCTCTGCGGTGAGGTACTCCAGGATGGCTGCGCTGTACACAGCGGCAGTCGCGCCCACACGTCCAT32740.26282643581182785No Hit
GTACATGGGAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT31290.2511862912813712No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG30170.24219528309232882No Hit
GGGTTGGACTGCAGTGGGTTTGGGGTCCTCAGGGACCAGGCAGAACTCGGTGTAGCATGCAGCCCAGATAATTTG28860.23167901458550247No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG28600.22959181625590336No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTG27260.21883471717258482No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA27030.2169883494194779TruSeq Adapter, Index 12 (96% over 29bp)
CAGGAAGGGAGCCAGAGACTGTGGCTCCCTTTGTACCAGCTGGTGAGGGGATGGACTGGCCCAGTGTGCCTCCTG25340.2034215602770836No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG25100.20149491566514593No Hit
TAACCTCCTTGGGCTTTACGAGGGCCTTGATAGCCTCGGCACGTGCACTCATGGCCTTGGCATTGTTGGCCTGCA24720.19844439502957803No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC22860.18351289928706122No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAA21980.1764485357099565TruSeq Adapter, Index 12 (96% over 29bp)
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA21560.1730769076390656No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC21160.1698658332858362No Hit
ACCCATACCAGCCACTGCAAAATCATGCCAAATTGTAAAGCCCATCGAGGCTAGGAAGAAAATGCATCAACTCTG21040.16890251097986736No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG20860.16745752752091414No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT20210.16223953169691632TruSeq Adapter, Index 12 (95% over 22bp)
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGG20100.16135648624977825No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA20010.1606339945203016No Hit
CAAGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCAT19180.15397101523735057No Hit
GGAAATCAGTGAATGAAGCCTCCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCTACA18620.1494755111428294No Hit
ATATAAAATAAAGCACTTAGAACCAAACCTGGAATATAAGTGCTTTGTTAAGTCAGAGCTTAAAAAAAGGAAATT18470.14827135826036836No Hit
GTACATGGGGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGC18210.14618415993076925No Hit
TAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG17260.1385578583418494No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC17020.13663121372991172TruSeq Adapter, Index 12 (95% over 23bp)
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGAT16880.13550733770628143No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG16550.13285820136486717No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAA16400.13165404848240614TruSeq Adapter, Index 12 (96% over 29bp)
CTCTTGGAATAGGCTCTTGCCAAAAAAACAAATTGAAGTTAGGTTTAATATGAGAAGTCAAAAATGATGTTATCC15370.12338553202284037No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC15190.12194054856388714No Hit
GCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATT15150.1216194411285642No Hit
CAGGGAGAATCGCTTGAACTCAGGAGGCAGAGGTCACAGTGAGCCAAGATCGCGCCATTGCACTCCAGCCTGGGC15030.12065611882259536No Hit
GTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCC14750.11840836677533477No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCC14660.11768587504585816No Hit
GTACATGGGGAGTAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT14650.11760559818702741No Hit
TATCACGCTTGCTTCTTCTTACTTCCCGAGCGCCAGCGCGATCAGGCCGAGCGTGCCGACAATGACGCGCCACCA14590.11712393703404302No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGT14590.11712393703404302No Hit
GCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC14410.11567895357508977No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAA14320.11495646184561314No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA14030.11262843293952184No Hit
AAATAGTACTTCCAATTCCAACTCAGTAGAATGATCTGTTTCTTGGGGGAAGTGCTTCAAGTGCTGTGACGGAAT13760.11046095775109196No Hit
CTACTTTCATCAGGTGATCCATACGTCTCTGTCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGTGCCCGG13610.10925680486863093No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGTC13450.10797237512733916No Hit
TCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGT13430.10781182140967771No Hit
TTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAA13150.10556406936241709No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGTCTT13040.10468102391527903No Hit
ATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAAACCTCACCCGGCCCG12630.10138967270321886No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA12630.10138967270321886No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCG12470.1001052429619271No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)