FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004046692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3263192
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG186550.5716795088980361No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC164730.5048124658310023No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG153180.46941767447333776No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCT131550.4031328833853478No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT123010.37696218916937774RNA PCR Primer, Index 47 (95% over 22bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT103790.3180628047629438No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC94960.29100341015790676No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA65890.20191885736420045No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT64140.19655601018879676RNA PCR Primer, Index 47 (96% over 28bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA63590.19487054393366984No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG61610.18880286541521307No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG55130.1689450084457182No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG49640.15212099073545168No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAA48060.14727910585708717RNA PCR Primer, Index 47 (96% over 29bp)
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAA46760.14329527652678725RNA PCR Primer, Index 47 (96% over 29bp)
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG46390.14216141740970192No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC45410.13915822299147584No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAA45050.13805500871539278No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGC43200.132385713129966No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG43140.13220184408395214No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAC42110.12904542546071454No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGC41770.1280035008666361No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC41090.1259196516784792No Hit
ATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCG37810.11586814382972255No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGC37780.11577620930671563No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA36860.11295688393450339No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAG35700.10940208237823579No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA35560.10897305460420348No Hit
ACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCG34460.10560212209394973No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC33760.10345698322378825RNA PCR Primer, Index 47 (95% over 23bp)
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAA32900.10082152689758984No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)