FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847453
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC41330.487696662823779TruSeq Adapter, Index 11 (95% over 21bp)
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG41230.4865166563809438No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCT37030.4369563857818664No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT33470.394948156416934TruSeq Adapter, Index 11 (95% over 23bp)
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAG21810.25735940518235234No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT19450.2295112531314421TruSeq Adapter, Index 11 (96% over 29bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG19050.22479122736010138No Hit
GGAGAATTCGGAACTACCAGAGGATGAAGATGAAGAGGAGAATGATGAAGATGTGGAGCAAGAAGTTCCCATAGA16460.1942290604906703No Hit
CCCCTGGGCTCAAAATCCACCTACCTTGGCCTACCAAAATGCTGGGATTACATGCATGAGCCACCACACTTGGCC14070.16602690650690954No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG12150.1433707828044741No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA11280.13310472675180807TruSeq Adapter, Index 11 (96% over 30bp)
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG11230.13251472353039045No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC11230.13251472353039045TruSeq Adapter, Index 11 (95% over 21bp)
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC11090.13086271451042122TruSeq Adapter, Index 11 (95% over 21bp)
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG11060.13050871257757068No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT10490.12378267585341017No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC10030.11835464621636833No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA9970.11764664235066724No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAA9730.1148146268878628TruSeq Adapter, Index 11 (96% over 30bp)
ATCTTAATGGGTACACAGTGCTTTAATTTGGCATCAATTATTAGCTGTTTTTTAAACAATTCATTATTACACCAG9570.11292661657932652No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC8860.10454857083519677TruSeq Adapter, Index 11 (95% over 24bp)
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT8780.10360456568092861TruSeq Adapter, Index 11 (95% over 23bp)
CCAGAGAATTCAGTACCTGCTTGGTCAGGAGGATGTGCACCATCTTGCCTTTGCACACCAGTCACCTGAACAAGG8740.10313256310379454No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG8660.1021885579495264No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)