FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957511
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCG253412.64654923024383No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCT205262.1436829446345786No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTG42190.4406215698827481No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTT39010.40741046316961377No Hit
CGTCAGATGTGTATAAGAGACAGGGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC38320.40020427963751853No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCCCTCCG34930.36479998663200736No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAAAAAAAAAAAAAAAAAAA33570.3505964944528052No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTAT29560.3087170800126578No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTT28240.29493133760343226No Hit
CGTCAGATGTGTATAAGAGACAGGCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGA27750.2898139029212197No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCA23550.24595017707368375No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG23550.24595017707368375No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGA23080.2410416172764595No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA19980.20866601010327818No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGT19970.20856157266078407No Hit
CCAGCTCCTCACCAACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAG19540.20407076263353635No Hit
GTGTATAAGAGACAGGGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGT19490.20354857542106566No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGGCGTG18840.196760141658947No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG18400.19216489418920513No Hit
GTATAAGAGACAGGCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA17540.1831832741347097No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGTT17440.18213889970976835No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTGGTT16750.17493271617767314No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGC16690.17430609152270837No Hit
GTATAAGAGACAGGGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTAT16610.1734705919827553No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTGTCTCTTATACACATCTCCGAGCCCA16490.17221734267282568No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCCCTCC16460.1719040303453433No Hit
GTGTATAAGAGACAGGCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGA16200.1691886568404958No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCCCAAAA16050.16762209520308383No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCC15920.1662644084506601No Hit
CGTCAGATGTGTATAAGAGACAGGGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAG15680.1637579098308009No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTAT15360.16041591167098862No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA14890.15550735187376435No Hit
CGTCAGATGTGTATAAGAGACAGCAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG14650.15300085325390517No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTGTCTCTTATACACATCTCCGAGCCCA14180.1480922934566809No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTC13880.14495917018185692No Hit
CGTCAGATGTGTATAAGAGACAGCCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCC13690.1429748587744684No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTAT13440.14036392271211506No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGA13300.1389017985171972No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAAAAAAAAAAAAAAAAAA12650.13211336475507854No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGGCG12540.13096455288764305No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAAAAAAAAAAAAAAAAAAAAAAAA12440.12992017846270174No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAAAAAAAAAAAAAAAAAAAAAAAAAAA12400.1295024286927252No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC12010.12542936843545402No Hit
GCAACTCATCCTTTGCACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATC11480.11989418398326494No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTTTGCCGTG11370.11874537211582947No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTA10670.11143475114124014No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTG10590.1105992516012871No Hit
GGTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAG10570.11039037671629881No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTGTCTCTTATACACATCTCCGAGCCCACGAGA10310.10767500321145135No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10290.10746612832646309No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT9920.10360194295418017TruSeq Adapter, Index 6 (95% over 21bp)
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC9820.10255756852923884No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)