FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004046710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51260
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGAAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGC4830.9422551697229808No Hit
CTGGAGAAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTAT3410.6652360515021459No Hit
CTGGAGAAGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCG2400.46820132657042524No Hit
CTGGAGAAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGG2010.3921186110027312No Hit
CTGGAGAAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGC1920.3745610612563402No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCGGCCGCCATTTGTCCGCATTGATCCCTG1720.3355442840421381No Hit
CTGGAGAAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTAT1710.333593445181428No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCTGTCTCTTATACACATCTCCGAGCCCAC1670.3257900897385876No Hit
CTGGAGAAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGG1340.26141240733515414No Hit
GCCTCAAACCCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTA1210.23605150214592274No Hit
CTGGAGAAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTAT1050.20483808037456108No Hit
CCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCTGTCTCTTATACACATCTCCGAGCCCACGA970.18923136948888022No Hit
CTGGAGAAGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCG940.1833788529067499No Hit
CTGGAGAAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGGCGTAT890.1736246586031994No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCT840.16387046429964885No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCGGCCGCCATTTGTCCGCATTGATCCAAG720.14046039797112758No Hit
CCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCGGCCGCCATTTGTCCGCATTGATCCCTGTC670.13070620366757707No Hit
CTGGAGAAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGC650.12680452594615685No Hit
CTGGAGAAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGG600.11705033164260631No Hit
CTGGAGAAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT600.11705033164260631Illumina PCR Primer Index 7 (96% over 26bp)
GCCTCAAACCCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG580.1131486539211861No Hit
CTGGAGAAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGGCGTATGC580.1131486539211861No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)