FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004046719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631681
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCT158452.5083863532384223No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGT72101.1413989022940376No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCG64861.0267840888043174No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGG45600.7218833556811112No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG42720.6762907226907252No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTG40590.6425711712082522No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCCCTCCG35400.5604094471734943No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTA32640.5167165072243743No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC26120.41349985198225053No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTG24320.3850044563632593No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACG21990.3481187498120095No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT21090.33387105200251393No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAT20350.3221562782480398No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTT18700.29603549893063114No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCCCTCC17700.28020472358674714No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGC16540.26184102418784166No Hit
CCAGCTCCTCACCAACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGT16070.2544005597762162No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCG15050.23825316892545448No Hit
CGTCAGATGTGTATAAGAGACAGGCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGA12560.19883453831918327No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTT11920.1887028420990975No Hit
GGTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAG11740.18585330253719837No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTCTT11580.18332037848217692No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTA11260.17825453037213404No Hit
CGTCAGATGTGTATAAGAGACAGGGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10810.17113068146738625No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT10480.16590652560390448No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG9930.15719959916476828No Hit
GCAACTCATCCTTTGCACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAC9540.15102559678065353No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCCCTCCGGATCC9370.14833436497219327No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTC9360.1481760572187544No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCCCATGT8420.13329512839550345No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCCCTCGG8260.130762204340482No Hit
GTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCG7800.12348004768229534No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGA7740.1225302011616623No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG7200.11398158247596492No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCC7170.11350665921564841No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTC6860.10859911885904436No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTG6750.10685773357121713No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCC6710.10622450255746176No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)