FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004046724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122687
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCT179680.8464743035595922No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC141120.6648177522168836No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG84970.40029453235451107No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT80040.37706925232029026RNA PCR Primer, Index 47 (95% over 22bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG76110.35855498243499867No Hit
GATGGGACGGTTCGAGACCGAGACGCTGGCTCTGGCCGGGAACCGTGCCGCGCTGGCCGACCTGAACGGACAATG71510.3368843357499245No Hit
CCTCCCAGGCTCCGCACCCCTGATGCTGCGCGGGTGCTGAGCCCGCTTCGGCCGGGACGATGGTGAAGTATTTCC67630.31860561637207935No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA67350.31728653353037917No Hit
CAGTGATCCATCCAAAAACAAGGACTGCAGCCTAAATTCCAAATACCAGAGACTGAAATTTTCAGCCTTGCTAAG64620.3044254758238025No Hit
CGCCCGCCCTGGGCGCCGGCGGCTCGGGGTCCACGGGTGGCGCCGTCGGGGGCAAGGGTGGCTCGGGGGTGGCCG61940.2917999686246724No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG53350.2513323914453709No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC43160.20332719802778268No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT42550.20045348183693593RNA PCR Primer, Index 47 (96% over 28bp)
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC41890.19734421513864264No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT38840.1829756341844087No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAA38120.17958370687717973RNA PCR Primer, Index 47 (96% over 29bp)
GCTCTCGCCTGCCTGCCCGCTCCCTTGCTTGCTCGCGCTTTCGCTCGCCCTCTCCTCGAGGATCGAGGGGACTCT38050.1792539361667547No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC35440.16695819967804956No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG34550.1627654006455026No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG31510.14844392979275794No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG31150.14674796613914345No Hit
CCCTACTGCAAAGAGAAGGTAGACCTCAAGAGGATGTTCAGCAATCCCTGGGAGAGGCCTCACGTCATGTATGGG27910.13148429325661296No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA25740.12126140123343666No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA25030.11791658402769697No Hit
AGTAGACGCAAGGCTCTCCTTTGATAAAGATGCGATGGTGGCCAGAGCCAGGCGGCTCATCGAGCTCCTGTCTCT22710.10698704048218131No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC21890.10312401216005941RNA PCR Primer, Index 47 (95% over 23bp)
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA21620.10185203941984852No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT21540.10147515860793418RNA PCR Primer, Index 47 (95% over 22bp)
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG21410.1008627272885734No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC21400.1008156171870841RNA PCR Primer, Index 47 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)