FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004046730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451880
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCT259205.736036115782952No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC171883.803664689740639TruSeq Adapter, Index 11 (95% over 21bp)
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT107362.3758519961051605TruSeq Adapter, Index 11 (95% over 23bp)
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA95592.1153846153846154No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG91782.0310701956271577No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC47931.0606798265026112TruSeq Adapter, Index 11 (95% over 21bp)
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT35260.7802956537133752No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC30110.6663273435425334TruSeq Adapter, Index 11 (95% over 21bp)
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTG28200.6240594848189785No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT25270.5592192617509073No Hit
CGTCAGATGTGTATAAGAGACAGGGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC20030.4432592723731964No Hit
CGTCAGATGTGTATAAGAGACAGGCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGA18010.3985571390634682No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG17140.37930424006373376No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACAT15760.3487651588917412No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCCCTCCG14880.3292909622023546No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAAAAAAAAAAAAAAAAAAA14500.32088164999557406No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCT14370.31800478003009647No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG13800.30539081171992566No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT12300.27219615827210764TruSeq Adapter, Index 11 (96% over 29bp)
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGA12230.2706470744445428No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC12100.26777020447906524TruSeq Adapter, Index 11 (95% over 24bp)
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCC11590.2564840223068071No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCCCAAAA11460.2536071523413296No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG11160.24696822165176596No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTGTCTCTTATACACATCTCCGAGCCCA10930.2418783747897672No Hit
CCAGCTCCTCACCAACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTA10260.22705142958307514No Hit
GTGTATAAGAGACAGGGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTAC9730.2153226520315128No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTGTCTCTTATACACATCTCCGAGCCCA9690.2144374612729043No Hit
CGTCAGATGTGTATAAGAGACAGGGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAG9580.212003186686731No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTG9570.21178188899707884No Hit
GTATAAGAGACAGGCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA9570.21178188899707884No Hit
GTGTATAAGAGACAGGCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGA9550.21133929361777465No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG9330.206470744445428No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTC8980.1987253253076038No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA8710.19275028768699656No Hit
CGTCAGATGTGTATAAGAGACAGCCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCC8580.18987341772151897No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTAC8530.1887669292732584No Hit
GGTTTGAGGCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC8300.18367708241125963No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCCCTCC8130.17991502168717358No Hit
CGTCAGATGTGTATAAGAGACAGCAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG8030.17770204479065238No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAA7820.17305479330795787TruSeq Adapter, Index 11 (96% over 30bp)
GTATAAGAGACAGGGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACAT7800.17261219792865362No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGA7200.15933433654952642No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCG7040.15579357351509251No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACAT6870.15203151279100646No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG6860.15181021510135434No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGC6800.1504824289634416No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGA6570.14539258210144285No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC6550.14494998672213863No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTGTCTCTTATACACATCTCCGAGCCCACGAGA6520.14428609365318226No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT6380.14118792599805258TruSeq Adapter, Index 11 (96% over 25bp)
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGC6260.13853235372222714No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGCTGTCTCTTAT6170.13654067451535806No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAAAAAAAAAAAAAAAAAA5990.1325573161016199No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT5980.1323360184119678TruSeq Adapter, Index 11 (95% over 23bp)
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAAAAAAAAAAAAAAAAAAAAAAAAAAA5920.13100823227405506No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTT5920.13100823227405506No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATGT5650.1250331946534478No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACG5640.1248118969637957No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTGTCTCTTAT5560.12304151544657872No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCCCAAA5340.1181729662742321No Hit
GCAACTCATCCTTTGCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACAT5280.11684518013631937No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACAT5250.11618128706736301No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAAAAAAAAAAAAAAAAAAAAAAAA5250.11618128706736301No Hit
GGTTTGAGGCCTGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT5110.11308311941223334No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG5080.11241922634327697No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACCTGTCTCTTATACACATCTCCG4880.10799327255023457No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCT4820.10666548641232185No Hit
CCAGCTCCTCACCAACACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA4760.10533770027440913No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG4670.10334602106754004No Hit
GCAACTCATCCTTTGCACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGC4600.10179693723997521No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTG4600.10179693723997521No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGA4590.10157563955032309No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAG4570.10113304417101886No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCAC4540.10046915110206249No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)