FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004046735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311066
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCT462313.5262145460259053No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCG150731.1496751498399012No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGT136091.03801029086255No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTG130700.9968987068538122No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTA112380.8571650855105692No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG103240.7874508224605016No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC80920.6172076768065071No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG76870.5863167834418709No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGG68060.5191195561474403No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTT65660.5008138415609893No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGTG59010.45009175739436463No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT57010.43483699523898867RNA PCR Primer, Index 41 (95% over 22bp)
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACAT53820.41050564960116426No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG39150.29861196919148236No Hit
CCAGCTCCTCACCAACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGA39030.29769668346215983No Hit
GCAACTCATCCTTTGCACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAA33280.2538392422654542No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCG30070.22935534900607596No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTT29910.22813496803364589No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTA29460.22470264654868635No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT29060.22165169411761118No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTG26950.20555792004368964No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTG26080.19892209850610112No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGTCTT25140.1917523602930745No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG24250.18496399113393222No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGTC23410.1785569910286744No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT22420.17100588376176332No Hit
GCGCAAACACCCTTCCTGTCTGCGTGATGGGAACCGCACCAGCACACGGGGCACGCGGAAGCCACTGCCGCAAGG21570.1645226098457286No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTA19920.1519374310675435No Hit
CCTCTGTACCATTGCCCAGTGGAAGCACGAGCAGCCCAGGCCGGGGCCGGGGTGAGGTGCGCCTGGAACTGGCTT19720.15041195485200592No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC18680.14247947853121049No Hit
GCAACTCATCCTTTGCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACAT17980.13714031177682892No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG17260.13164859740089363No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTT16690.1273009901866115No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC14910.11372425186832698No Hit
GGTTTGAGGCCTGTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATG14740.11242759708512005No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCT13680.10434257314277084No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)