FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976342
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCG110841.135257932159018No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCT51160.5239967142661075No Hit
CATTCCACTCGTGTTGATTCCATCCCTTTCCATTCCATTCGGTTTGAAGGCATTCCATTCCATTCCATTCCATTC18560.19009732245463168No Hit
GCAGGGAGGGGCATTGAGACTGCGTCCCATAAAGCTGAAAGAAGAGGGAGTGGAGCACGCTGGACCATGCCCCCA18550.18999489932830915No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG18080.1851810123911498No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT16970.17381204536934802No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT16610.17012481282173664No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA12420.12720952289259296No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCAT11460.11737690276562925No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTT10700.10959274516511633No Hit
GTACATGGGAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGG10630.10887578328085855No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)