FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186822
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCT152441.2844386100021739No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC91510.7710507557156844No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA53360.4496040686808974No Hit
ATGGTAAAGATGTATTTCAAGCAAAAATTGCCTGTATGGCAAAAACGCTGGAGAGTCTGAAAGGAAGGAGTGAGT52310.44075691215700413No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT48910.4121089767463023RNA PCR Primer, Index 47 (95% over 22bp)
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG39860.3358549133736988No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC25970.21881967135762564No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC23260.19598558166262506No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG19730.16624228401563168No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG19680.16582099084782723No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG18840.15874326562871263No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC17990.1515812817760372No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT16050.13523510686522494No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT15510.130685140652937RNA PCR Primer, Index 47 (96% over 28bp)
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT15070.12697776077625794No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA14130.1190574492215345No Hit
GAGTCTCCCTCTGTTGCCCAGGCTGGAGTGCAGTGGTGCGATCTCGGCTCACTGCAACCTCTGCCTCCTGGGTTC13760.11593987977978164No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAA13210.1113056549339328RNA PCR Primer, Index 47 (96% over 29bp)
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC12330.10389089518057466RNA PCR Primer, Index 47 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)