FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004046751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64779
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGAAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGC4260.6576205251701941No Hit
CTGGAGAAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTAT3000.46311304589450286No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCTGTCTCTTATACACATCTCCGAGCCCAC2350.3627718859506939No Hit
CTGGAGAAGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCG1870.2886737986075734No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCGGCCGCCATTTGTCCGCATTGATCCCTG1780.2747804072307384No Hit
CCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCTGTCTCTTATACACATCTCCGAGCCCACGA1480.22846910264128806No Hit
CTGGAGAAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGC1470.2269253924883064No Hit
CTGGAGAAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTAT1430.22075055187637968No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCT1300.2006823198876179No Hit
CTGGAGAAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGG1300.2006823198876179No Hit
CTGGAGAAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGG1250.19296376912270952No Hit
CCTCGAGTGGAAAAGCAGAAGACACGTTGAAAAAACATAGGAACAATCCCACAATCCAAGAGAACCAAACCTGCC1200.18524521835780114No Hit
GCCTCAAACCCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTA1190.18370150820481945No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTGTCTCTTATACACATCTCCGAGCCCA840.12967165285046078No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCGGCCGCCATTTGTCCGCATTGATCCAAG760.11732197162660739No Hit
CCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCGGCCGCCATTTGTCCGCATTGATCCCTGTC760.11732197162660739No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGT750.11577826147362572No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG750.11577826147362572No Hit
CTGGAGAAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGC740.11423455132064403No Hit
CTGGAGAAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTAT730.11269084116766236No Hit
CTTGAAGTTATGCGGCCGCCATTTGTCCGCATTGATCCAAGCGCTTGACTTCGGCCGCCGCCGCGAGCGGCGGCC720.11114713101468068No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGA700.10805971070871734No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCTGTCTCTTATACACATCTCCGAGCCCACGAGA670.1034285802497723No Hit
CTGGAGAAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGGCGTAT670.1034285802497723No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)