FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919641
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCG210462.288501708818985No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCT79510.8645765032224532No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT27840.3027268249240736No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG24270.2639073290555771No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTT21100.22943735653369085No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCAT19310.20997323955760996No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGTT18890.20540624004366922No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA16190.17604695745405No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTT15390.1673479107608295No Hit
CCAGCTCCTCACCAACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTG14790.1608236257409141No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTG13910.15125467437837156No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCCCTCCG10940.11895946352979044No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATGG10680.11613227335449378No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCG10210.1110215834222267No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGT9650.10493225073697236No Hit
GCAACTCATCCTTTGCACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGC9250.10058272739036211No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)